Exposure assessment of phthalate esters in Japanese pregnant women by using urinary metabolite analysis
Exposure assessment of phthalate esters in Japanese pregnant women by using urinary metabolite analysis
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DOI：
10.1007/s12199-009-0078-9
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发表时间：
2009-05-01
影响因子：
4.7
通讯作者：
Shiraishi, Hiroaki
中科院分区：
文献类型：
--
作者：
Suzuki, Yayoi;Niwa, Mayu;Shiraishi, Hiroaki
我们的目的是(1)评估单点尿是否是长期邻苯二甲酸酯暴露评估的合适媒介，(2)使用尿液代谢物作为其后代产前暴露水平的指标来估计日本孕妇的邻苯二甲酸酯摄入量。采用高效液相色谱-串联质谱（HPLC-MS/MS）分析了50例孕妇尿液中7种邻苯二甲酸酯类的9种代谢物（MMP、MEP、MnBP、MBzP、MEHP、MEOHP、MEHHP、MINP、MnOP）。我们从50名孕妇中收集了12名受试者，每名受试者在5-12周内采集了4份尿液样本，通过计算类内相关系数（ICC）比较了尿代谢物的个体内和个体间差异。我们根据尿液代谢物浓度估计了50名孕妇的每日摄入量。12例孕妇单点尿液中7种邻苯二甲酸酯代谢物浓度的ICCs分别为：MMP（0.57）、MEP（0.47）、MnBP（0.69）、MBzP（0.28）、MEHP（0.51）、MEHHP（0.43）和MEOHP（0.41）。邻苯二甲酸酯代谢物在50例受试者中检出率较高。日平均摄食量为0.01 ~ 2 μ g/kg / d。所有受试者的日摄入水平均低于欧洲食品安全局（EFSA）规定的相应的可耐受日摄入量（TDI），但DnBP的最大值为6.91 μ g/kg /天，占TDI值的70%。较高的icc表明，单点尿液中邻苯二甲酸酯代谢物水平可能反映受试者长期暴露于相应的糖尿病。虽然目前的暴露水平低于tdi，但需要进一步的研究和暴露监测来揭示邻苯二甲酸酯对敏感亚群的毒性。
Our objectives were (1) to evaluate whether single spot urine is suitable media for longer-term phthalate esters exposure assessment, and (2) to estimate intake level of phthalate esters of Japanese pregnant women using urinary metabolites as an indicator of prenatal exposure level in their offspring.We analyzed nine metabolites (MMP, MEP, MnBP, MBzP, MEHP, MEOHP, MEHHP, MINP, MnOP) of seven phthalate esters in spot urine samples from 50 pregnant women by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Using four urine samples collected from each of 12 subjects from 50 pregnant women within 5-12 weeks, we compared intra- and interindividual variation in urinary metabolites by calculation of intraclass correlation coefficient (ICC). We estimated daily intakes of 50 pregnant women from their urinary metabolite concentrations.ICCs for seven phthalate metabolite concentrations in single spot urine samples were: MMP (0.57), MEP (0.47), MnBP (0.69), MBzP (0.28), MEHP (0.51), MEHHP (0.43), and MEOHP (0.41) in 12 pregnant women. Phthalate ester metabolites had high detection rates in 50 subjects. The mean daily intake ranged from 0.01 to 2 mu g/kg per day. The daily intake levels in all subjects were lower than corresponding tolerable daily intake (TDI) set by the European Food Safety Authority (EFSA), though maximum value for DnBP of 6.91 mu g/kg per day accounted for 70% of TDI value.Higher ICCs indicated that phthalate metabolite levels in single spot urine could reflect longer-term exposure to the corresponding diesters of subjects. Although the current exposure level was less than TDIs, further studies and exposure monitoring are needed to reveal the toxicity of phthalate esters to sensitive subpopulation.