Quantitative proteomics analysis of differentially expressed proteins in ruptured and unruptured cerebral aneurysms by iTRAQ
Quantitative proteomics analysis of differentially expressed proteins in ruptured and unruptured cerebral aneurysms by iTRAQ
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iTRAQ 对破裂和未破裂脑动脉瘤差异表达蛋白的定量蛋白质组学分析
DOI：
10.1016/j.jprot.2018.05.001
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发表时间：
2018-06-30
影响因子：
3.3
通讯作者：
Wang, Shuo
中科院分区：
文献类型：
--
作者：
Jiang, Pengjun;Wu, Jun;Wang, Shuo
脑动脉瘤破裂的潜在病理生理机制尚不清楚。本研究利用定量蛋白质组学方法研究破裂和未破裂动脉瘤之间的差异表达蛋白质。手术中收集了6个破裂动脉瘤和6个未破裂动脉瘤的瘤壁。使用相对和绝对定量等压标签(ITRAQ)鉴定差异表达的蛋白质，并通过Western blotting验证目的蛋白质的表达。利用KEGG数据库和GO数据库对差异表达蛋白进行生物信息学分析。在破裂和未破裂的动脉瘤中，169个蛋白质有不同的表达，其中74个蛋白质表达上调，95个蛋白质表达下调，其变化倍数为>；=2和p值。
The underlying pathophysiological mechanisms involved in cerebral aneurysms rupture remain unclear. This study was performed to investigate the differentially expressed proteins between ruptured and unruptured aneurysms using quantitative proteomics. The aneurysmal walls of six ruptured aneurysms and six unruptured aneurysms were collected during the surgical operation. The isobaric tags for relative and absolute quantification (iTRAQ) were used to identify the differentially expressed proteins and western blotting was performed to validate the expression of the proteins of interest. Bioinformatics analysis of the differentially expressed proteins was also performed using the KEGG database and GO database. Between ruptured and unruptured aneurysms, 169 proteins were found differently expressed, including 74 up-regulated proteins and 95 down-regulated proteins with a fold change >= 2 and p value