Influence of carvacrol on proliferation and survival of porcine lymphocytes and intestinal epithelial cells in vitro
Influence of carvacrol on proliferation and survival of porcine lymphocytes and intestinal epithelial cells in vitro
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DOI：
10.1016/j.tiv.2007.11.023
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发表时间：
2008-04-01
影响因子：
3.2
通讯作者：
Rothkoetter, Hermann-Josef
中科院分区：
文献类型：
--
作者：
Bimczok, Diane;Rau, Henriette;Rothkoetter, Hermann-Josef
香芹酚是牛至和百里香的精油化合物，在猪营养中作为抗生素生长促进剂的替代品具有潜在的应用前景。香芹酚以其抗菌作用而闻名，但目前尚不清楚是否对猪免疫系统有额外的影响。本文研究了香芹酚对猪外周血淋巴细胞的影响。检查猪肠上皮细胞系IPEC-1以进行比较。香芹酚抑制纯化的淋巴细胞的增殖，在MTT测定中IC 50为182 +/- 67 μ M。这通过CFSE测定证实。香芹酚处理的淋巴细胞制剂中单核细胞的存在对淋巴细胞具有保护作用，显著提高IC 50至516 +/- 87 μ M。CFSE标记的淋巴细胞亚群的FACS分析显示，γ δ T细胞对香芹酚毒性的敏感性低于CD 4和CD 8 T细胞。香芹酚暴露后测得的淋巴细胞增殖减少被证明是由于凋亡细胞死亡，如通过膜联蛋白-V结合和半胱天冬酶-3活化所确定的。所观察到的效果是不特定的淋巴细胞，因为香芹酚同样诱导细胞凋亡和抑制猪肠上皮细胞系IPEC-1的增殖。(C)2008爱思唯尔有限公司保留所有权利。
Carvacrol, an essential oil compound of oregano and thyme, has potential applications as an alternative to antibiotic growth promoters in pig nutrition. Carvacrol is well known for its antibacterial effects, but it is unclear whether there are additional effects on the porcine immune system. In the present study, the influence of carvacrol on porcine blood lymphocytes was examined. The porcine enterocyte cell line IPEC-1 was examined for comparison. Carvacrol inhibited the proliferation of purified lymphocytes with an IC50 of 182 +/- 67 mu M in MTT assays. This was confirmed by CFSE assay. The presence of monocytes in carvacrol-treated lymphocyte preparations had a protective effect on the lymphocytes, significantly raising the IC50 to 516 +/- 87 mu M. FACS analysis of CFSE labelled lymphocyte subsets revealed that gamma delta T cells were less susceptible to carvacrol toxicity than CD4 and CD8 T cells. The reduced lymphocyte proliferation measured after carvacrol exposure was shown to be due to apoptotic cell death, as determined by annexin-V binding and caspase-3 activation. The observed effects were not specific for lymphocytes, since carvacrol similarly induced apoptosis and suppressed proliferation in the porcine enterocyte cell line IPEC-1. (C) 2008 Elsevier Ltd. All rights reserved.