Development of a Portable SPR Sensor for Nucleic Acid Detection
Development of a Portable SPR Sensor for Nucleic Acid Detection
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DOI：
10.3390/mi11050526
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发表时间：
2020-05-01
期刊：
影响因子：
3.4
通讯作者：
Yu, Duli
中科院分区：
文献类型：
--
作者：
Huang, Yafeng;Zhang, Lulu;Yu, Duli
核酸检测在临床诊断、环境监测、食品安全等方面具有重要意义。与传统的核酸扩增检测方法相比，表面等离子体共振（SPR）传感技术具有无标记、操作简单、实时检测等优点。然而，在许多SPR角度调制检测应用中，角度扫描系统通常需要高分辨率的步进电机和复杂的机械结构来调节角度。设计了一种便携式多角度扫描SPR传感器。传感器仅用一个步进电机带动皮带转动，皮带拉动入射光和反射光的机械杆向相反方向运动，实现入射角和反射角相等的SPR角扫描方式。该传感器的角度扫描精度为0.002度，响应灵敏度为3.72 × 10(-6) RIU（折射率单位），角度扫描范围为30度-74度。系统整体尺寸仅为480mm × 150mm × 180mm。采用便携式SPR传感器在牛血清白蛋白修饰的金膜芯片上检测核酸杂交。结果表明，该传感器灵敏度高，响应速度快，可成功完成0.01 μ mol/mL目标DNA溶液的杂交检测。
Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002 degrees, response sensitivity of 3.72 x 10(-6) RIU (refractive index unit), and an angle scanning range of 30 degrees-74 degrees. The overall size of the system is only 480 mm x 150 mm x 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 mu mol/mL.