Rice stripe virus p2 Colocalizes and Interacts with Arabidopsis Cajal Bodies and Its Domains in Plant Cells
Rice stripe virus p2 Colocalizes and Interacts with Arabidopsis Cajal Bodies and Its Domains in Plant Cells
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DOI：
10.1155/2020/5182164
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发表时间：
2020-06
影响因子：
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通讯作者：
Luping Zheng;Pengxiang Hong;Xiaonan Guo;Yang Li;Li-qiong Xie
中科院分区：
文献类型：
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作者：
Luping Zheng;Pengxiang Hong;Xiaonan Guo;Yang Li;Li-qiong Xie
水稻条纹病毒的p2可能从细胞核转移到细胞质，并募集核仁功能，促进病毒的系统运动。Cajal小体是与核仁相连的核组分，在植物病毒侵染中起重要作用。卷曲蛋白（Coilin）是CB的标志蛋白，对CB的形成和功能起重要作用。卷曲蛋白包含三个结构域，N-末端、中心和C-末端片段。使用酵母双杂交，共定位，和双分子荧光互补（BiFC）的方法，我们表明，p2与全长的拟南芥coilin（Atcoilin），中心和C-末端结构域Atcoilin在细胞核中的相互作用。此外，N-末端是Atcoilin与Cajal体相互作用所不可或缺的。
p2 of rice stripe virus may translocate from the nucleus to the cytoplasm and recruit nucleolar functions to promote virus systemic movement. Cajal bodies (CBs) are nuclear components associated with the nucleolus, which play a major role in plant virus infection. Coilin, a marker protein of CBs, is essential for CB formation and function. Coilin contains three domains, the N-terminal, the center, and the C-terminal fragments. Using yeast two-hybrid, colocalization, and bimolecular fluorescence complementation (BiFC) approaches, we show that p2 interacts with the full-length of Arabidopsis thaliana coilin (Atcoilin), the center and C-terminal domain of Atcoilin in the nucleus. Moreover, the N-terminal is indispensable for Atcoilin to interact with Cajal bodies.