Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity
Bioaerosol Sampler Choice Should Consider Efficiency and Ability of Samplers To Cover Microbial Diversity
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DOI：
10.1128/aem.01589-18
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发表时间：
2018-12-01
影响因子：
4.4
通讯作者：
Duchaine, Caroline
中科院分区：
文献类型：
--
作者：
Mbareche, Hamza;Veiltette, Marc;Duchaine, Caroline
生物气溶胶研究的目的是描述微生物的含量，增加对与疾病有关的气溶胶化过程的了解。空气采样器用于收集、识别和量化生物气溶胶。比较空气采样器性能的研究通常使用培养方法或在实验室环境中针对特定微生物。本研究的目的是使用环境领域的样品，比较效率的3个高气流速率采样器描述生物气溶胶的多样性，使用下一代测序方法。两个液体旋流冲击器和一个静电过滤器干采样器被用于在四个污水处理厂，以针对细菌的多样性和在五个奶牛场，以针对真菌的多样性。干式静电采样器在收集更多的真菌和细菌操作分类单位（OTU）方面始终更强大。液体和干样品之间的OTU丰度的显着差异被揭示。干静电采样所揭示的大多数多样性并没有使用旋流液体撞击器进行识别。这项工作的结果表明，生物气溶胶采样器的选择应包括采样器覆盖微生物多样性的效率和能力的信息。虽然这些结果表明，静电过滤器导致更好的覆盖率的微生物多样性之间的测试空气采样器，需要进一步的研究来证实这一假设。虽然这是很难确定一个普遍最佳的空气采样器，这项工作提供了一个深入的了解，在选择空气采样器研究bioaerosols. IMPORTANCE微生物生态学时，一些必不可少的考虑因素相关联的生物气溶胶暴露和健康问题是具有挑战性的，充分的暴露监测是科学家在该领域的优先事项。从生物气溶胶接触研究中得出的结论在很大程度上取决于研究的设计和所使用的方法。空气采样策略是准确解释空气中存在的物质的第一个方法步骤。应用新的分子方法来评估在该领域使用的不同类型的采样器的效率是必要的，以规避传统的方法和偏见，他们引入这样的研究。在进行生物气溶胶研究时，应考虑本文提供的结果和结论。
Bioaerosol studies aim to describe the microbial content and increase understanding of the aerosolization processes linked to diseases. Air samplers are used to collect, identify, and quantify bioaerosols. Studies comparing the performances of air samplers have typically used a culture approach or have targeted a specific microorganism in laboratory settings. The objective of this study was to use environmental field samples to compare the efficiencies of 3 high-airflow-rate samplers for describing bioaerosol diversity using a next-generation sequencing approach. Two liquid cyclonic impactors and one electrostatic filter dry sampler were used in four wastewater treatment plants to target bacterial diversity and in five dairy farms to target fungal diversity. The dry electrostatic sampler was consistently more powerful in collecting more fungal and bacterial operational taxonomic units (OTUs). Substantial differences in OTU abundances between liquid and dry sampling were revealed. The majority of the diversity revealed by dry electrostatic sampling was not identified using the cyclonic liquid impactors. The findings from this work suggest that the choice of a bioaerosol sampler should include information about the efficiency and ability of samplers to cover microbial diversity. Although these results suggest that electrostatic filters result in better coverage of the microbial diversity among the tested air samplers, further studies are needed to confirm this hypothesis. While it is difficult to determine a single universally optimal air sampler, this work provides an in-depth look at some of the considerations that are essential when choosing an air sampler for studying the microbial ecology of bioaerosols.IMPORTANCE Associating bioaerosol exposure and health problems is challenging, and adequate exposure monitoring is a priority for scientists in the field. Conclusions that can be drawn from bioaerosol exposure studies are highly dependent on the design of the study and the methodologies used. The air sampling strategy is the first methodological step leading to an accurate interpretation of what is present in the air. Applying new molecular approaches to evaluate the efficiencies of the different types of samplers used in the field is necessary in order to circumvent traditional approaches and the biases they introduce to such studies. The results and conclusions provided in this paper should be taken in consideration when conducting a bioaerosol study.