A semiautomated approach for artefact removal in serial tissue cryosections.
A semiautomated approach for artefact removal in serial tissue cryosections.
复制标题
连续组织冷冻切片中伪影去除的半自动化方法。
DOI：
10.1111/j.1365-2818.2010.03424.x
复制
发表时间：
2011
影响因子：
2
通讯作者：
Carson,JP
中科院分区：
文献类型：
--
作者：
Kindle,LM;Kakadiaris,IA;Ju,T;Carson,JP
用光学显微镜可以对器官的连续组织切片进行成像，其分辨率可以详细描述单个细胞。当组织切片首先进行原位杂交或免疫组织化学时，可以分析这些数据集的基因表达和基因产物的变化。这些空间信息对于理解实验或环境挑战对生物体的功能影响非常重要。然而，分析这些数据集的关键步骤是减轻组织切片制备过程中产生的伪影。在本文中，我们描述了一种带有手动验证工具的自动化方法，这些工具可以一起检测和处理包括灰尘颗粒和气泡在内的人工制品。
Thinly sliced serial tissue sections of an organ can be imaged using optical microscopy at a resolution detailing individual cells. When the tissue sections are first subjected toin situhybridization or immunohistochemistry, these data sets can be analysed for changes in gene expression and gene products. Such spatial information is important for understanding the functional effects of experimental or environmental challenges to the organism. However, a critical step in analysing these data sets is mitigating artefacts that result from the preparation of the tissue sections. In this paper, we describe an automated method with manual validation tools that together enable detecting and addressing artefacts including dust particles and air bubbles.