Calcitonin gene-related peptide and substance P immunoreactivity in rat trigeminal ganglia and brainstem following adjuvant-induced inflammation of the temporomandibular joint
Calcitonin gene-related peptide and substance P immunoreactivity in rat trigeminal ganglia and brainstem following adjuvant-induced inflammation of the temporomandibular joint
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DOI：
10.1016/s0003-9969(99)00129-6
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发表时间：
2000-04-01
影响因子：
3
通讯作者：
Harper, RP
中科院分区：
文献类型：
--
作者：
Hutchins, B;Spears, R;Harper, RP
用免疫组织化学方法检测大鼠三叉神经节和脑干中降钙素基因相关肽(CGRP)和P物质的免疫反应性，以探讨佐剂性炎症在不同的急性(6、24和48 h)和中期(10 D)时的变化。将佐剂相关神经肽的浓度与对侧赋形剂相关组织和未注射对照的浓度进行比较。注射后6h，三叉神经节内CGRP免疫反应强度明显高于对侧脑组织。降钙素基因相关肽在以下各时间点均有所下降。但在第10天仍显著升高。注射侧神经节内P物质在4个时间段均显著升高。在脑干尾侧亚核，CGRP在4个时间段均显著升高。P物质在尾侧亚核的免疫反应在最初的三个时间段显著增加，但到第10天时已下降到对照组的水平。这些数据表明，P物质和CGRP在诱导炎症后神经肽的变化模式是不同的。此外，脑干和三叉神经节的变化模式也不同。这表明这两种神经肽在炎症过程中可能具有不同的作用，这一过程可能受到脑干和神经节不同机制的调节。(C)2000爱思唯尔科学有限公司。保留所有权利。
The immunoreactivity of two inflammatory mediators, calcitonin gene-related peptide (CGRP) and substance P, was measured in the trigeminal ganglia and brainstem to characterize an adjuvant-induced inflammation within the rat temporomandibular joint at various acute (6, 24 and 48 h) and inter-mediate (10 day) time intervals. Concentrations of adjuvant-related neuropeptides were compared to those in both contralateral vehicle-related tissues and non-injected controls. By 6 h, CGRP immunoreactivity in the trigeminal ganglia was significantly above that in contralateral vehicle-injected tissue. The CGRP had decreased at each of the following time-points. but remained significantly elevated at 10 days. Substance P in the ganglion on the injected side was significantly increased for all four time periods. In brainstem subnucleus caudalis, CGRP was significantly increased for all four time periods. Substance P immunoreactivity in the subnucleus caudalis was significantly increased for the initial three time periods, but by day 10 had been reduced to that of the control. These data show that the pattern of changes in neuropeptides following the induction of inflammation is different between substance P and CGRP. Moreover, the pattern of change varies between the brainstem and the trigeminal ganglion. This suggests that the two neuropeptides may have different roles in the inflammatory process, and that this process may be modulated by different mechanisms at the brainstem and ganglion. (C) 2000 Elsevier Science Ltd. All rights reserved.