ELEVATION OF TRANSFORMING GROWTH-FACTOR-BETA-1 LEVEL IN CEREBROSPINAL-FLUID OF PATIENTS WITH COMMUNICATING HYDROCEPHALUS AFTER SUBARACHNOID HEMORRHAGE
ELEVATION OF TRANSFORMING GROWTH-FACTOR-BETA-1 LEVEL IN CEREBROSPINAL-FLUID OF PATIENTS WITH COMMUNICATING HYDROCEPHALUS AFTER SUBARACHNOID HEMORRHAGE
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DOI：
10.1161/01.str.25.7.1400
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发表时间：
1994-07-01
期刊：
影响因子：
8.3
通讯作者：
TADA, T
中科院分区：
文献类型：
--
作者：
KITAZAWA, K;TADA, T
背景与目的转化生长因子- β 1 （tgf - β 1）是一种控制细胞外基质蛋白生成的多功能多肽。血小板储存了大量的tgf - 1，在出血时释放出来。我们最近报道了人重组tgf - β 1诱导小鼠通讯性脑积水。本研究的目的是确定tgf - β 1是否与蛛网膜下腔出血（SAH）后交通性脑积水的发生有关。方法采用酶联免疫吸附法测定24例SAH患者脑脊液中tgf - β 1的含量。比较脑积水组和非脑积水组的水平。Western blot检测脑脊液中tgf - β 1活性。结果tgf - β 1在SAH发病后迅速下降。13例脑室扩张伴脑室周围低密度ct扫描患者的tgf - β 1水平在第12 ~ 14天为1.07+/-0.37 ng/mL，显著高于无脑室扩张患者的0.52+/-0.21 ng/mL （P < 0.05）
Background and Purpose Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional polypeptide that controls the production of extracellular matrix protein. Platelets store a large quantity of TGF-beta 1, which is released at hemorrhage. We recently reported that human recombinant TGF-beta 1 induced communicating hydrocephalus in mice. The aim of this study was to determine whether TGF-beta 1 is related to the development of communicating hydrocephalus after subarachnoid hemorrhage (SAH).Methods TGF-beta 1 in the cerebrospinal fluid of 24 patients with SAH was measured with enzyme-linked immunosorbent assay. The levels were compared between hydrocephalic and nonhydrocephalic groups. Western blot analysis was performed to determine active TGF-beta 1 in the cerebrospinal fluid.Results TGF-beta 1 rapidly decreased from the onset of SAH. The level of TGF-beta 1 of 13 patients showing ventricular dilatation with periventricular low density on computed tomographic scan was 1.07+/-0.37 ng/mL on days 12 through 14, which was significantly higher than 0.52+/-0.21 ng/mL in patients without ventricular dilatation (P