Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning
Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning
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DOI：
10.1101/gad.11.9.1160
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发表时间：
1997-05-01
影响因子：
10.5
通讯作者：
Errington, J
中科院分区：
文献类型：
--
作者：
Glaser, P;Sharpe, ME;Errington, J
枯草芽孢杆菌的spo0J基因在生长和芽孢形成过程中对染色体的精确分配是必需的。我们通过免疫荧光法以及在活细胞中利用spo0J - gfp融合蛋白对Spo0J蛋白的亚细胞定位进行了表征。我们发现Spo0J蛋白形成离散的稳定焦点，通常位于靠近细胞两极的位置。这些焦点与新一轮DNA复制的起始协同复制，之后子代焦点在细胞内迁移分开。这种迁移与细胞长度的延伸无关，并且可能是为了将子代染色体导向细胞的相反两极，为细胞分裂做准备。在芽孢形成过程中，焦点移动到细胞的极端两极，在那里它们的作用是使染色体的oriC区域定位，为极性隔膜形成做好准备。这些观察结果为细菌中存在一种负责染色体分配的动态的、类似有丝分裂的装置提供了有力证据。
The Bacillus subtilis spo0J gene is required for accurate chromosome partitioning during growth and sporulation. We have characterized the subcellular localization of Spo0J protein by immunofluorescence and, in living cells, by use of a spo0J-gfp fusion. We show that the Spo0J protein forms discrete stable foci usually located close to the cell poles. The foci replicate in concert with the initiation of new rounds of DNA replication, after which the daughter foci migrate apart inside the cell. This migration is independent of cell length extension, and presumably serves to direct the daughter chromosomes toward opposite Doles of the cell, ready for division. During sporulation, the foci move to the extreme poles of the cell, where they function to position the oriC region of the chromosome ready for polar septation. These observations provide strong evidence for the existence of a dynamic, mitotic-like apparatus responsible for chromosome partitioning in bacteria.