Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice.
Generation and characterization of Lhx3GFP reporter knockin and Lhx3loxP conditional knockout mice.
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Lhx3GFP 报告基因敲入和 Lhx3loxP 条件敲除小鼠的生成和表征。
DOI：
10.1002/dvg.23098
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Gan,Lin
中科院分区：
文献类型：
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作者：
Xu,Mei;Xie,Xiaoling;Dong,Xuhui;Liang,Guoqing;Gan,Lin
LHX 3是一种LIM同源域转录因子，广泛表达于发育中的垂体、脊髓、髓质、视网膜和内耳，在胚胎发育过程中发挥重要作用。Lhx 3基因缺失突变小鼠垂体发育障碍，围产期死亡。为了促进Lhx 3在小鼠中的功能研究，我们设计并表征了两种新的Lhx 3小鼠品系：Lhx 3GFP报告基因敲入和Lhx 3loxP条件性敲除小鼠。LHX 3和GFP的共免疫标记显示敲入GFP报告基因的表达模式重现了耳蜗、前庭、视网膜和脊髓中内源性LHX 3的表达模式。通过将Lhx 3loxP小鼠与普遍存在的CMV-Cremice杂交，我们已经证明了Cre重组酶介导的Lhx 3外显子3至5的高效去除，其编码LHX 3的第二个LIM结构域和HD结构域，导致Lhx 3的整体敲除。因此，Lhx 3GFP和Lhx 3loxP小鼠作为有价值的遗传工具来剖析Lhx 3在小鼠妊娠晚期和出生后阶段的组织特异性作用。
LHX3, a LIM‐homeodomain transcription factor, is broadly expressed in the developing pituitary, spinal cord, medulla, retina and inner ear, and plays essential roles during embryonic development. Mice with homozygousLhx3null mutation exhibit failure in the formation of pituitary gland and die perinatally. To facilitate the functional study ofLhx3in mice, we engineered and characterized two novelLhx3mouse strains:Lhx3GFPreporter knock‐in andLhx3loxPconditional knockout mice. Coimmunolabeling of LHX3 and GFP shows that the expression pattern of the knock‐in GFP reporter recapitulates that of endogenous LHX3 in cochlea, vestibule, retina, and spinal cord. By crossingLhx3loxPmice with the ubiquitousCMV‐Cremice, we have demonstrated a high efficiency of Cre recombinase‐mediated removal of exons 3 to 5 ofLhx3, which encode the second LIM‐domain and the HD domain of LHX3, resulting global knockout ofLhx3. Thus,Lhx3GFPandLhx3loxPmice serve as valuable genetic tools to dissect the tissue‐specific roles ofLhx3at late‐gestation and postnatal stages in mice.