Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media
Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media
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DOI：
10.1038/ncomms1646
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发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Rohrbach, Alexander
中科院分区：
文献类型：
--
作者：
Fahrbach, Florian O.;Rohrbach, Alexander
即使在存在巨大的位相扰动的情况下，激光也能自我重建其初始光束轮廓，从而能够更深入地传播到非均匀介质中。对于厚层介质，如细胞团、胚胎、皮肤或脑组织或植物，以及散射合成材料，这种能力对于基于光片的显微镜具有至关重要的优势。围绕贝塞尔光束中心强度最大值的环形系统使其能够自我重建，但同时照亮了焦外区域并降低了图像对比度。在这里，我们提出了一种检测方法，将环系统的负面影响降至最低。光束沿一条直线的传输稳定性允许使用共焦线原理，导致图像对比度显著增加。与使用扫描高斯光束的标准光片技术相比，轴向分辨率可以提高近100%，同时在高传播深度下也表现出自重构。
Laser beams that can self-reconstruct their initial beam profile even in the presence of massive phase perturbations are able to propagate deeper into inhomogeneous media. This ability has crucial advantages for light sheet-based microscopy in thick media, such as cell clusters, embryos, skin or brain tissue or plants, as well as scattering synthetic materials. A ring system around the central intensity maximum of a Bessel beam enables its self-reconstruction, but at the same time illuminates out-of-focus regions and deteriorates image contrast. Here we present a detection method that minimizes the negative effect of the ring system. The beam's propagation stability along one straight line enables the use of a confocal line principle, resulting in a significant increase in image contrast. The axial resolution could be improved by nearly 100% relative to the standard light-sheet techniques using scanned Gaussian beams, while demonstrating self-reconstruction also for high propagation depths.