Simple and versatile selection of Arabidopsis transformants
Simple and versatile selection of Arabidopsis transformants
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DOI：
10.1007/s00299-002-0473-9
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发表时间：
2002-08-01
影响因子：
6.2
通讯作者：
Reiss, B
中科院分区：
文献类型：
--
作者：
Hadi, MZ;Kemper, E;Reiss, B
在无菌条件下进行植物转化和组织培养获得的大量拟南芥种子的选择比较费工、费时和昂贵。我们在这里描述了一种替代的选择程序，它使用常规的温室条件和功能，并使用各种选择性试剂。该筛选系统以惰性生长底物Grodan为基础，对卡那霉素、潮霉素、BASTA、磺胺嘧啶和甲氨蝶呤转基因拟南芥种子进行了密集的筛选试验。然而，该系统有能力适用于更具选择性的试剂和更广泛的植物物种。对于拟南芥，选择性试剂的效果与组织培养程序相当或更好。
The selection of large amounts of seeds of Arabidopsis thaliana obtained after in planta transformation under sterile conditions and in tissue culture is relatively labour-intensive, time-consuming and expensive. We describe here an alternative selection procedure that uses regular greenhouse conditions and functions with a wide variety of selective agents. The selection system is based on the inert growth substrate Grodan and was intensively tested for the selection of transgenic Arabidopsis seeds with kanamycin, hygromycin, BASTA, sulfadiazine and methotrexate. However, the system has the capacity to be applicable for more selective agents and to a wider range of plant species. For Arabidopsis, the effectiveness of selective agents is comparable to or better than that obtained with tissue culture procedures.