Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons
Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons
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DOI：
10.1073/pnas.93.18.9844
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发表时间：
1996-09-03
影响因子：
11.1
通讯作者：
Dichter, MA
中科院分区：
文献类型：
--
作者：
Cao, YX;Wilcox, KS;Dichter, MA
通过单细胞mRNA扩增和PCR检测，在生理学上鉴定为GABA能抑制性或谷氨酸能兴奋性的极低密度海马培养物中的神经元均含有γ-氨基丁酸（GABA）合成酶、谷氨酸脱羧酶（GAD）的mRNA。然而，与生理学一致，免疫细胞化学显示，只有一个子集的神经元染色的GAD蛋白或GABA。类似的部分与GAD 65和GAD 67的RNA探针杂交。海马CA 1锥体神经元切片制备，这是传统上认为是兴奋性的，也含有GAD 65和GAD 67的mRNA。培养的海马神经元不含其他两种神经递质合成酶，酪氨酸羟化酶和胆碱乙酰转移酶的mRNA。这些数据表明，在一些神经元，可能是兴奋性神经元，GAD mRNA的翻译水平上的选择性调节。我们建议，神经递质的表型可能是转录后调节和神经元可能会表现出短暂的表型可塑性，以应对环境的影响。
Neurons in very low density hippocampal cultures that are physiologically identified as either GABAergic inhibitory or glutamatergic excitatory all contain mRNA for the gamma-aminobutyric acid (GABA) synthetic enzyme, glutamic acid decarboxylase (GAD), as detected by single cell mRNA amplification and PCR. However, consistent with the physiology, immunocytochemistry revealed that only a subset of the neurons stain for either GAD protein or GABA. A similar fraction hybridize with RNA probes for GAD65 and GAD67. Hippocampal CA1 pyramidal neurons in slice preparations, which are traditionally thought to be excitatory, also contain mRNA for GAD65 and GAD67. Hippocampal neurons in culture did not contain mRNA for two other neurotransmitter synthesizing enzymes, tyrosine hydroxylase, and choline acetyl transferase. These data suggest that in some neurons, presumably the excitatory neurons, GAD mRNA is selectively regulated at the level of translation. We propose that neurotransmitter phenotype may be posttranscriptionally regulated and neurons may exhibit transient phenotypic plasticity in response to environmental influences.