Sphingosine‐1‐phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells
Sphingosine‐1‐phosphate induces a Ca2+ signal in primary rat astrocytes and a Ca2+ signal and shape changes in C6 rat glioma cells
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1-磷酸鞘氨醇在原代大鼠星形胶质细胞中诱导 Ca2+ 信号，在 C6 大鼠神经胶质瘤细胞中诱导 Ca2+ 信号和形状变化
DOI：
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发表时间：
1998
影响因子：
4.2
通讯作者：
K. Koschel
中科院分区：
文献类型：
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作者：
Piet W.L. Tas;K. Koschel
用β-肾上腺素能激动剂L-异丙肾上腺素处理大鼠神经胶质瘤C6细胞导致cAMP水平升高，随后细胞形态从上皮型改变为星形胶质细胞型。通过添加EC 50为10 nM的1-磷酸鞘氨醇（S1 P），可逆转这种形态学反应。在加载有Ca 2+指示剂Fura-2的大鼠胶质瘤C6细胞中，S1 P诱发Ca 2+从内部储存释放和Ca 2+从外部介质流入。Ca 2+增加的半数最大刺激为10-20 nM。在负载Ca 2+指示剂fluo-3的原代大鼠星形胶质细胞中观察到类似的Ca 2+信号。用PMA（162 nM）预处理C6细胞可防止S1 P-诱导的Ca 2+升高和形态学逆转。因此，Ca 2+离子似乎是必不可少的形态逆转S1 P。用肉毒梭菌C3外切酶预处理细胞不影响S1 P对形态学反应的逆转，表明小GTP结合蛋白Rho不参与S1 P诱导的逆转。神经科学杂志Res. 52：427-434，1998.© 1998 Wiley利斯公司
Treatment of rat glioma C6 cells with the β‐adrenergic agonist L‐isoproterenol leads to a rise in cAMP level and a subsequent change in cell morphology from an epithelial to an astrocytic type of appearance. This morphological response is reverted by the addition of sphingosine‐1‐phosphate (S1P) with an EC50 of 10 nM. In rat glioma C6 cells loaded with the Ca2+ indicator Fura‐2, S1P evoked Ca2+ release from internal stores and Ca2+ influx from the external medium. Half‐maximal stimulation of the Ca2+ increase was 10–20 nM. A similar Ca2+ signal was observed in primary rat astrocytes loaded with the Ca2+ indicator fluo‐3. Pretreatment of the C6 cells with PMA (162 nM) prevented both the S1P‐induced Ca2+ increase and the morphological reversion. Ca2+ ions therefore seem essential for the morphological reversion by S1P. Pretreatment of the cells with the Clostridium botulinum C3 exoenzyme did not affect the reversion of the morphological response by S1P, indicating that the small GTP‐binding protein Rho is not involved in the S1P‐induced reversion. J. Neurosci. Res. 52:427–434, 1998. © 1998 Wiley‐Liss, Inc.