Molecular Cloning and Characterization of a Geranyl Diphosphate-Specific Aromatic Prenyltransferase from Lemon
Molecular Cloning and Characterization of a Geranyl Diphosphate-Specific Aromatic Prenyltransferase from Lemon
复制标题
DOI：
10.1104/pp.114.246892
复制
发表时间：
2014-09-01
期刊：
影响因子：
7.4
通讯作者：
Yazaki, Kazufumi
中科院分区：
文献类型：
--
作者：
Munakata, Ryosuke;Inoue, Tsuyoshi;Yazaki, Kazufumi
异戊二烯残基赋予不同的生物活性，例如对酚类化合物（包括黄酮类、香豆素和氧杂蒽酮）的抗病原体和抗食草活性。迄今为止，已分离出约 1,000 种异戊二烯化酚类化合物，这些化合物含有各种异戊二烯基残基。然而，目前描述的所有植物异戊二烯基转移酶（PT）均已显示对作为异戊二烯基供体的二甲基烯丙基二磷酸具有特异性，而大多数编码这些基因的互补DNA已从豆科植物中分离出来。在这项研究中，我们描述了从柠檬（Citrus limon）中鉴定出的一种新型 PT 基因 ClPT1，属于尿黑酸 PT 家族。该基因编码的PT在多肽序列上不同于其他已知的PT，包括类黄酮特异性PT。这种膜结合酶对作为异戊二烯基供体的香叶基二磷酸和作为异戊二烯基受体的香豆素具有特异性。此外，该基因产物靶向植物细胞中的质体。据我们所知，这是柑橘属植物中特有的二磷酸香叶酯的新型芳香族 PT。
Prenyl residues confer divergent biological activities such as antipathogenic and antiherbivorous activities on phenolic compounds, including flavonoids, coumarins, and xanthones. To date, about 1,000 prenylated phenolics have been isolated, with these compounds containing various prenyl residues. However, all currently described plant prenyltransferases (PTs) have been shown specific for dimethylallyl diphosphate as the prenyl donor, while most of the complementary DNAs encoding these genes have been isolated from the Leguminosae. In this study, we describe the identification of a novel PT gene from lemon (Citrus limon), ClPT1, belonging to the homogentisate PT family. This gene encodes a PT that differs from other known PTs, including flavonoid-specific PTs, in polypeptide sequence. This membrane-bound enzyme was specific for geranyl diphosphate as the prenyl donor and coumarin as the prenyl acceptor. Moreover, the gene product was targeted to plastid in plant cells. To our knowledge, this is the novel aromatic PT specific to geranyl diphosphate from citrus species.