Quantification of genes encoding resistance to aminoglycosides, β-lactams and tetracyclines in wastewater environments by real-time PCR
Quantification of genes encoding resistance to aminoglycosides, β-lactams and tetracyclines in wastewater environments by real-time PCR
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DOI：
10.1080/09603120802449593
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Lindgren, Per-Eric
中科院分区：
文献类型：
--
作者：
Borjesson, Stefan;Dienues, Olaf;Lindgren, Per-Eric
在本研究中，开发了基于 LUX (TM) 技术的实时 PCR 测定，用于对介导氨基糖苷类 [aac(6')-Ie + aph(2'')]、β-内酰胺 (mecA) 和四环素（tetA 和 tetB）耐药性的基因进行定量，用于废水环境。所开发的检测方法适用于从三种废水相关环境中提取的 DNA：来自处理垃圾填埋场渗滤液的地表流动区域的土壤、来自城市废水处理厂的生物膜以及来自医院废水管道的污泥。在医院管道中观察到所有基因的浓度最高，在地表水流系统中最低。 TetA 和 aac(6')-Ie + aph(2 '') 可以在所有环境中检测到。在地表径流区和医院废水管道中检测到tetB基因，在废水处理厂和医院管道中检测到mecA基因。开发的 LUX (TM) 实时 PCR 检测被证明是快速且可重复的工具，用于检测和量化废水中编码抗生素耐药性的四种基因。
In this study real-time PCR assays, based on the LUX (TM)-technique, were developed for quantification of genes mediating resistance to aminoglycosides [aac(6')-Ie + aph(2 '')], beta-lactams (mecA), and tetracyclines (tetA and tetB), for use in wastewater environments. The developed assays were applied on DNA extracted from three wastewater-associated environments: soil from an overland flow area treating landfill leachates, biofilm from a municipal wastewater treatment plant, and sludge from a hospital wastewater pipeline. The highest concentration of all genes was observed in the hospital pipeline and the lowest in the overland flow system. TetA and aac(6')-Ie + aph(2 '') could be detected in all environments. The tetB gene was detected in the overland flow area and the hospital wastewater pipeline and mecA was detected in the wastewater treatment plant and the hospital pipeline. The developed LUX (TM) real-time PCR assays were shown to be fast and reproducible tools for detection and quantification of the four genes encoding antibiotic resistance in wastewater.