Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies
Expression of stem cell markers nestin and cytokeratin 15 and 19 in cutaneous malignancies
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DOI：
10.1111/j.1468-3083.2010.03791.x
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发表时间：
2011-03-01
影响因子：
9.2
通讯作者：
Bhawan, J.
中科院分区：
文献类型：
--
作者：
Abbas, O.;Bhawan, J.
背景新的证据表明干细胞参与了不同皮肤肿瘤的发病机制。干细胞标记物的免疫组化使用促进了干细胞鉴定。虽然很少有研究检查细胞角蛋白（CK）15和细胞角蛋白（CK）19在基底细胞癌（BCC）和鳞状细胞癌（SCC）的表达，只有很少有巢蛋白表达在这两种恶性肿瘤进行了检查。另外，干细胞标记物的表达，据我们所知，还没有在默克尔细胞癌（MCC）中进行检测。方法在本研究中，我们检测了51例MCC中干细胞标记物CK15，CK19和nestin的表达（MCC 11例，BCC和SCC各20例）从皮肤病理学实验室的病理档案中检索，结果30%的基底细胞癌和5%的鳞状细胞癌细胞角蛋白15阳性，而所有MCC细胞角蛋白15均为阴性。60%的基底细胞癌和30%的鳞状细胞癌CK19呈阳性表达，而所有MCC均呈点状阳性表达。结论干细胞标志物CK15、CK19和nestin在基底细胞癌、鳞状细胞癌和MCC中的表达谱基本一致。除了证实以前的报告的结果，我们的研究还显示，在大约一半的SCC病例中，nestin表达阳性，这增加了这种恶性肿瘤的皮肤肿瘤，迄今为止已报道表现出nestin表达。
Background Emerging evidence is implicating stem cells in the pathogenesis of different cutaneous neoplasms. The immunohistochemical use of stem cell markers has facilitated stem cell identification. While few studies have examined the expression of cytokertatin (CK) 15 and cytokeratin (CK) 19 in basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), only rarely have nestin expression been examined in these two malignancies. Furthermore, stem cell marker expression, to the best of our knowledge, has not been examined in Merkel cell carcinoma (MCC).Methods In this study, we examined the expression of stem cell markers CK15, CK19 and nestin in 51 overall cases (11 cases of MCC and 20 cases each of BCC and SCC) retrieved from the pathology files of Skin Pathology Laboratory, Boston University School of Medicine.Results Cytokeratin 15 immunoreactivity was observed in 30% of BCC cases and only a single (5%) SCC case, while all MCC cases were negative. While 60% of BCC cases and 30% of SCC cases stained positively with CK19, all MCC cases showed positive immunostaining in a dot-like pattern. While negative in BCC and MCC, nestin expression was interestingly seen in 45% of SCC cases.Conclusions Our study described the expression profiles of stem cell markers CK15, CK19 and nestin in BCC, SCC and MCC. In addition to confirming results of the previous reports, our study also showed positive nestin expression in around half of SCC cases, which adds this malignancy to the cutaneous tumours that have been so far reported to exhibit nestin expression.