IDENTIFICATION OF GLYCEROPHOSPHORYLCHOLINE IN MUSSEL OVARIAN EXTRACTS BY 2-DIMENSIONAL NUCLEAR-MAGNETIC-RESONANCE
IDENTIFICATION OF GLYCEROPHOSPHORYLCHOLINE IN MUSSEL OVARIAN EXTRACTS BY 2-DIMENSIONAL NUCLEAR-MAGNETIC-RESONANCE
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DOI：
10.1016/0003-2697(92)90362-b
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发表时间：
1992-11-01
影响因子：
2.9
通讯作者：
LANE, AN
中科院分区：
文献类型：
--
作者：
FAN, TWM;LANE, AN
在分化或增殖组织（包括生殖器官和肿瘤）中，“磷酸二酯”峰值的丰度值得进一步研究其代谢作用，这将需要严格确认其身份。在生物样品的31p核磁共振光谱中，该峰对甘油磷酸胆碱的分配主要基于化学位移，这可能导致歧义。我们采用了二维31p1H异核位移相关和1H全相关光谱的组合来追踪甘油磷酸胆碱的自旋连度，从而直接从贻贝的粗卵巢提取物中鉴定其结构，而没有歧义，也不需要大量的纯化。该方法可广泛应用于组织粗提取物中含杂原子分子的鉴定。
The abundance of the “phosphodiester” peak in differentiating or proliferating tissues, including reproductive organs and tumors, warrants further investigations of its metabolic role(s), which would require a rigorous confirmation of its identity. The assignment of this peak to glycerophosphorylcholine in31P NMR spectra of biological samples has been largely based on chemical shift, which can result in ambiguities. We employed a combination of two-dimensional31P1H heteronuclear shift correlation and1H total correlation spectroscopies to trace the spin connectivities of glycerophosphorylcholine and thus to identify its structure directly from crude ovarian extracts of mussels without ambiguities and the need for extensive purification. This approach can be applied generally to the identification of molecules containing heteroatoms in crude tissue extracts.