Examination of the brain mitochondrial lipidome using shotgun lipidomics.
Examination of the brain mitochondrial lipidome using shotgun lipidomics.
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DOI：
10.1007/978-1-60761-322-0_1
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Seyfried, Thomas N
中科院分区：
文献类型：
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作者：
Kiebish, Michael A;Han, Xianlin;Seyfried, Thomas N
亚细胞器和髓磷脂的污染阻碍了对脑线粒体脂质组进行表征的尝试。要准确测量线粒体脂质的含量和分子种类组成，需要高度纯净的线粒体。我们设计了一种不连续的菲可（Ficoll）和蔗糖梯度法，用于分离和纯化无任何可检测到污染的脑线粒体。鸟枪法脂质组学被用于分析脑线粒体的脂质组成。这些方法可用于确定内在的脂质异常是否是与神经和神经退行性疾病相关的线粒体功能障碍的基础。
Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.