Identification and functional analysis of novel and conserved microRNAs in tomato
Identification and functional analysis of novel and conserved microRNAs in tomato
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DOI：
10.1007/s11033-014-3410-4
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发表时间：
2014-05
影响因子：
2.8
通讯作者：
Yushi Luan;Weichen Wang;Ping Liu
中科院分区：
文献类型：
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作者：
Yushi Luan;Weichen Wang;Ping Liu
MicroRNA 是约 22 nt 非编码内源 RNA，在多个物种中发挥重要的调节作用。通过使用基于同源性的计算研究，从 EST、GSS 和核苷酸序列中鉴定出属于 10 个家族的 14 个新颖且保守的番茄 miRNA。这些 miRNA 的实时 PCR 分析随后证明了它们在番茄中的表达。同时，使用 psRNATarget 总共预测了 10 个 miRNA 的 36 个潜在靶点。靶基因主要涉及代谢、跨膜运输、应激反应和转录调控。根据我们的实验，在生物和非生物胁迫下，miR398有不同程度的下调，表明miR398可能参与番茄胁迫调控网络。我们的结果补充了番茄 miRNA 的发现，并且还表明 miRNA 在应激反应中的关键调节功能。
MicroRNAs are ~22 nt non-coding endogenous RNAs which play important regulation roles in various species. By using homology-based computational research, 14 novel and conserved tomato miRNAs belonging to ten families were identified from EST, GSS, and nucleotide sequences. Real-time PCR analysis of these miRNAs demonstrated their expression in tomato afterwards. Meanwhile, a total of 36 potential targets were predicted for the ten miRNAs using psRNATarget. The target genes were mainly involved in metabolism, transmembrane transport, stress response, and transcription regulation. According to our experiment, miR398 was down-regulated on different levels under biotic and abiotic stresses, suggesting that miR398 might be involved in tomato stress regulatory network. Our results supplement the findings of tomato miRNAs and also suggest crucial regulatory functions of miRNAs in stress responses.