Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium
Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium
复制标题
DOI：
10.1016/j.ibiod.2006.07.008
复制
发表时间：
2006-09-01
影响因子：
4.8
通讯作者：
Qiao, C. L.
中科院分区：
文献类型：
--
作者：
Lan, W. S.;Gu, J. D.;Qiao, C. L.
农药的降解通常是有益的，因为破坏农药的反应将环境中的大多数农药残留物转化为无活性、毒性较低、无害的化合物。本文介绍了一种利用共表达载体培养能解毒不同农药的细菌的新策略。设计了pETDuet载体，用于同时表达两个目的基因。在共表达载体上克隆了黄杆菌的有机磷酸水解酶基因（opd）和库蚊的羧酸酯酶B1基因（B1）。表达的OPH和B1酶的分子量分别约为35和65 kDa。一种微生物能够产生降解有机磷、碳酸盐和拟除虫菊酯农药的两种酶。具有更多酶的基因工程细菌的技术能力将为将来扩大可生物降解农药的范围开辟新的机会。(c) 2006 Elsevier Ltd.版权所有。
Degradation of pesticides is usually beneficial, since the reactions that destroy pesticides convert most pesticide residues in the environment to inactive, less toxic, harmless compounds. This paper describes a novel strategy using a coexpression vector for the purpose of developing bacteria that can detoxify different pesticides. The vector pETDuet was designed for coexpression of two target genes simultaneously. The organophosphate hydrolase gene (opd) from Flavobacterium sp. and carboxylesterase B1 gene (b1) from Culex pipiens were cloned in the coexpression vector. The expressed enzymes of OPH and B1 had a molecular mass of approximately 35 and 65 kDa, respectively. A single microorganism was capable of producing both enzymes for degradation of organophosphorus, carbarnate and pyrethroid pesticides. The technical capability of genetically engineering bacteria with more enzymes should open up new opportunities for extending the wide range of pesticides that can be biodegraded in the future. (c) 2006 Elsevier Ltd. All rights reserved.