Hepatitis B Virus-Infected HepG2hNTCP Cells Serve as a Novel Immunological Tool To Analyze the Antiviral Efficacy of CD8+ T Cells In Vitro
Hepatitis B Virus-Infected HepG2hNTCP Cells Serve as a Novel Immunological Tool To Analyze the Antiviral Efficacy of CD8+ T Cells In Vitro
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DOI：
10.1128/jvi.00605-15
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发表时间：
2015-07-01
影响因子：
5.4
通讯作者：
Thimme, Robert
中科院分区：
文献类型：
--
作者：
Hoh, Alexander;Heeg, Maximilian;Thimme, Robert
CD8(+) T细胞是控制乙肝病毒（HBV）感染的主要效应淋巴细胞。然而，模型系统的局限性，如低感染率，限制了hbv特异性CD8(+) T细胞的机制研究。在这里，我们建立了一种基于hbv感染的HepG2（hNTCP）细胞的新型免疫细胞培养模型，该模型内源性处理病毒抗原并将其提交给hbv特异性CD8(+) T细胞。这诱导了细胞溶解和非细胞溶解CD8(+) t细胞效应功能和病毒载量的减少。
CD8(+) T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8(+) T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2(hNTCP) cells that endogenously processed viral antigens and presented them to HBV-specific CD8(+) T cells. This induced cytolytic and noncytolytic CD8(+) T-cell effector functions and reduction of viral loads.