Extraction, Purification, and Chromatographic Analysis of Bile Acids in Biological Materials
Extraction, Purification, and Chromatographic Analysis of Bile Acids in Biological Materials
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生物材料中胆汁酸的提取、纯化和色谱分析
DOI：
10.1007/978-1-4757-0647-5_5
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. Sjövall
中科院分区：
文献类型：
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作者：
P. Eneroth;J. Sjövall
近年来出现了几篇涉及胆汁酸分析不同方面的综述（1-15）。这些文章涵盖了广泛的技术，特别强调薄层和气相色谱。在本章中，我们将尝试介绍已建立的胆汁酸分析方法，因为它们可以组合成一个完整的分析程序，包括提取，纯化，鉴定和定量。不可能包括所有方法和已发表的标准程序的各种修改。在评论（1-15）中可以找到对其中大多数的参考。Sobotka关于胆汁酸和类固醇的书中描述了该领域的早期发展（16,17）。每当存在同样有效或类似的方法时，我们通常更愿意描述我们个人经验的方法。许多用于胆汁酸的技术与用于类固醇分析的技术相似，这一领域的进展与胆汁酸领域的工作者高度相关。
Several reviews covering different aspects of bile acid analysis have appeared in recent years (1–15). These articles cover a wide range of techniques with particular emphasis on thin-layer and gas chromatography. In this chapter we will try to present established methods for bile acid analysis as they can be combined to form a complete analytical procedure, including extraction, purification, identification, and quantitation. It has not been possible to include all methods and the various modifications of standard procedures that have been published. References to most of these can be found in the reviews (1–15). Early development in the field is described in Sobotka’s books on bile acids and steroids (16, 17). Whenever equally efficient or similar methods exist we have generally preferred to describe those of which we have personal experience. Many of the techniques used for bile acids are similar to those used in steroid analysis and advance in this area is highly relevant for workers in the bile acid field.