Cimetidine and granulopoiesis: bone marrow culture studies in normal man and patients with cimetidine-associated neutropenia.
Cimetidine and granulopoiesis: bone marrow culture studies in normal man and patients with cimetidine-associated neutropenia.
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西咪替丁和粒细胞生成：正常人和西咪替丁相关中性粒细胞减少症患者的骨髓培养研究。
DOI：
10.1111/j.1365-2141.1980.tb05982.x
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发表时间：
1980
影响因子：
6.5
通讯作者：
Koeffler,HP
中科院分区：
文献类型：
--
作者：
Fitchen,JH;Koeffler,HP
我们研究了H2受体拮抗剂西咪替丁和甲硫胺对7名正常志愿者和2名西咪替丁相关性中性粒细胞减少症患者骨髓细胞体外髓系集落形成的影响。浓度为500μg/ml的西咪替丁对正常粒-巨噬细胞集落形成的抑制率为50%，1000μg/ml的西咪替丁则完全抑制细胞增殖。甲硫胺在较低浓度时的抑制率分别为50%(250μg/ml)和95%(350μg/ml)，西咪替丁对两例西咪替丁相关性中性粒细胞减少症患者骨髓集落形成的抑制作用相似。用患者的急性期血清和西咪替丁治疗自体和异体骨髓，没有显示出抗体介导的粒细胞生成抑制的证据。我们的结果表明，在足够高的浓度下，西咪替丁和甲硫胺在体外抑制人骨髓髓系集落形成。这些发现与H2受体拮抗剂可能通过损伤体内粒系祖细胞而以剂量相关的方式产生中性粒细胞减少的假设一致。
SummaryWe studied the effect of the H2‐receptor antagonists, cimetidine and metiamide, onin vitromyeloid colony formation by bone marrow cells from seven normal volunteers and two patients with a cimetidine‐associated neutropenia. A cimetidine concentration of 500 μg/ml produced 50% inhibition of normal granulocyte—macrophage colony formation, and 1000 μg/ml of cimetidine completely suppressed proliferation. The inhibitory effect of metiamide occurred at lower concentrations: 50% inhibition at 250 μg/ml and 95% inhibition at 350 μg/ml. Cimetidine had a similar inhibitory effect on colony formation by recovery marrow from the two patients with cimetidine‐associated neutropenia. Treatment of autologous and allogeneic marrows with patients’ acute phase sera and cimetidine failed to show evidence of antibody‐mediated suppression of granulopoiesis. Our results indicate that at sufficiently high concentrations, cimetidine and metiamide inhibit human bone marrow myeloid colony formationin vitro. These findings are consistent with the hypothesis that H2‐receptor antagonists may produce neutropenia in a dose‐related fashion by injury to granulocytic progenitor cellsin vivo.