Expression of Bcl-2 and Bax in mouse renal tubules during kidney development.
Expression of Bcl-2 and Bax in mouse renal tubules during kidney development.
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肾脏发育过程中小鼠肾小管中 Bcl-2 和 Bax 的表达
DOI：
10.1371/journal.pone.0032771
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhai XY
中科院分区：
文献类型：
--
作者：
Song XF;Ren H;Andreasen A;Thomsen JS;Zhai XY
Bcl-2和Bax在细胞凋亡调节以及肾脏形态发生过程中的细胞粘附和迁移中发挥重要作用，其结构和功能与线粒体相关。为了阐明 Bcl-2 和 Bax 在肾脏发育过程中的作用，有必要确定它们在肾脏中表达的确切位置。本研究定位了它们在肾脏发育过程中的表达。将来自胚胎 (E) 16、17、18 天龄小鼠胎儿和出生后 (P) 1、3、5、7、14、21 天幼鼠的肾脏包埋在 Epon 中。对来自两个 E17 肾脏的半薄连续切片进行了计算机辅助 3D 肾小管追踪。该追踪技术与新开发的免疫组织化学技术相结合，可以对戊二醛固定的塑料包埋切片进行免疫组织化学分析。因此，可以详细描述微观结构，并且可以使用完全相同的切片进行免疫化学。研究表明，Bcl-2和Bax在所有时间点在成熟的近曲小管中均强表达，在近曲小管中表达较弱，在未成熟的近曲小管和远端小管中仅弱表达。输尿管芽和其他早期发育结构如逗号体、S形体、肾小球等中未检测到表达。偶尔观察到仅表达Bcl-2的小管。本研究表明，在肾脏发育过程中，Bcl-2 和 Bax 在近端肾小管和远端肾小管中的表达不同，尽管这两个肾小管段几乎同样配备有线粒体。 Bcl-2 和 Bax 在近端和远端肾小管中不同表达的功能意义尚不清楚。然而，本研究的结果表明，成熟近端小管和其余小管之间的线粒体功能有所不同。 Bcl-2 和 Bax 在肾小管发生过程中的功能仍需研究。
Bcl-2 and Bax play an important role in apoptosis regulation, as well as in cell adhesion and migration during kidney morphogenesis, which is structurally and functionally related to mitochondria. In order to elucidate the role of Bcl-2 and Bax during kidney development, it is essential to establish the exact location of their expression in the kidney. The present study localized their expression during kidney development. Kidneys from embryonic (E) 16-, 17-, 18-day-old mouse fetuses, and postnatal (P) 1-, 3-, 5-, 7-, 14-, 21-day-old pups were embedded in Epon. Semi-thin serial sections from two E17 kidneys underwent computer assisted 3D tubule tracing. The tracing was combined with a newly developed immunohistochemical technique, which enables immunohistochemistry on glutaraldehyde fixated plastic embedded sections. Thereby, the microstructure could be described in detail, and the immunochemistry can be performed using exactly the same sections. The study showed that Bcl-2 and Bax were strongly expressed in mature proximal convoluted tubules at all time points, less strongly expressed in proximal straight tubules, and only weakly in immature proximal tubules and distal tubules. No expression was detected in ureteric bud and other earlier developing structures, such as comma bodies, S shaped bodies, glomeruli, etc. Tubules expressing Bcl-2 only were occasionally observed. The present study showed that, during kidney development, Bcl-2 and Bax are expressed differently in the proximal and distal tubules, although these two tubule segments are almost equally equipped with mitochondria. The functional significance of the different expression of Bcl-2 and Bax in proximal and distal tubules is unknown. However, the findings of the present study suggest that the mitochondrial function differs between mature proximal tubules and in the rest of the tubules. The function of Bcl-2 and Bax during tubulogenesis still needs to be investigated.
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影响因子：
5.3
作者：
Fedorov, Lev M.;Schmittwolf, Carolin;Kneitzf, Burkhard
通讯作者：
Kneitzf, Burkhard
影响因子：
2
作者：
Messaris, Evangelos;Memos, Nikolaos;Bramis, John
通讯作者：
Bramis, John
影响因子：
2.4
作者：
Foley, JGD;Bard, JBL
通讯作者：
Bard, JBL
影响因子：
--
作者：
RUEGG, CE;MANDEL, LJ
通讯作者：
MANDEL, LJ
DOI：
10.1152/ajprenal.1997.272.5.f640
发表时间：
1997-05-01
影响因子：
4.2
作者：
Basile, DP;Liapis, H;Hammerman, MR
通讯作者：
Hammerman, MR