The effect of local anesthetics administered via pain pump on chondrocyte viability
The effect of local anesthetics administered via pain pump on chondrocyte viability
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DOI：
10.1177/0363546508318190
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发表时间：
2008-08-01
影响因子：
4.8
通讯作者：
Wood, Billy
中科院分区：
文献类型：
--
作者：
Dragoo, Jason L.;Korotkova, Tatiana;Wood, Billy
背景资料：软骨溶解术开始由术后，关节内疼痛pumps最近已被描述由multiple institutions.Purpose：To evaluate the in vitro chondrocytoxicity of anesthesia formulations commonly used in pain pumps.Study Design：Controlled laboratory study.Methods：新鲜分离的人关节软骨细胞培养24-，48-，和72-hour trials在一个定制的生物反应器，模仿滑液的代谢。将软骨细胞灌注在Dulbecco改良Eagle培养基10%胎牛血清和以下药物之一中：1%利多卡因、1%利多卡因与肾上腺素、0.25%布比卡因、0.25%布比卡因与肾上腺素、0.5%布比卡因或0.5%布比卡因与肾上腺素。使用具有生长培养基的静态和灌注培养物作为对照。所有实验一式两份进行。进行活/死染色，并通过荧光显微镜和histomorphometry.Results：死亡：活细胞的比例进行了评估：在所有时间点的药物含有肾上腺素（P <0.05）的所有培养物中发现了显着更多的软骨细胞坏死。与对照组相比，0.25%和0.5%布比卡因在24和48小时显示出相似的坏死率。然而，0.5%布比卡因在72小时产生显著更多的细胞死亡。与1%利多卡因相比，在24 hours.Conclusion：在这种体外模型中，0.25%和0.5%布比卡因造成最小的软骨细胞坏死时，用于疼痛泵最多48小时，表现出类似的坏死率。所有含肾上腺素（pH
Background: Chondrolysis initiated by postoperative, intra-articular pain pumps has recently been described by multiple institutions.Purpose: To evaluate the in vitro chondrotoxicity of anesthetic formulations commonly used in pain pumps.Study Design: Controlled laboratory study.Methods: Freshly isolated human articular chondrocytes were cultured for 24-, 48-, and 72-hour trials in a custom bioreactor that mimics the metabolism of synovial fluid. Chondrocytes were perfused in Dulbecco's Modified Eagle's Medium 10% fetal bovine serum and one of the following medications: 1% lidocaine, 1% lidocaine with epinephrine, 0.25% bupivacaine, 0.25% bupivacaine with epinephrine, 0.5% bupivacaine, or 0.5% bupivacaine with epinephrine. Static and perfusion cultures with growth media were used as controls. All experiments were run in duplicate. Live/dead staining was performed, and the ratio of dead: live cells was assessed by fluorescent microscopy and histomorphometry.Results: Significantly more chondrocyte necrosis was found in all cultures with medications containing epinephrine (P < .05) at all time points. Similar necrosis rates were exhibited in 0.25% and 0.5% bupivacaine compared with controls at 24 and 48 hours. However, 0.5% bupivacaine produced significantly more cell death at 72 hours. Similar necrosis rates were exhibited with 1% lidocaine compared to controls at 24 hours.Conclusion: In this in vitro model, 0.25% and 0.5% bupivacaine caused minimal chondrocyte necrosis when used in pain pumps for a maximum of 48 hours. All anesthetics containing epinephrine (pH