A light microscopic and ultrastructural study on the presence and location of oxytocin in the equine endometrium
A light microscopic and ultrastructural study on the presence and location of oxytocin in the equine endometrium
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DOI：
10.1016/s0093-691x(02)01362-6
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发表时间：
2003-09-15
期刊：
影响因子：
2.8
通讯作者：
Watson, ED
中科院分区：
文献类型：
--
作者：
Bae, SE;Watson, ED
据报道，催产素不仅在下丘脑和垂体后叶中产生，而且在经典的下丘脑-神经垂体轴以外的部位如卵巢、睾丸、胎盘和一些非生殖部位也产生催产素。在母马的子宫内膜中发现了催产素mRNA，在子宫中发现了催产素及其神经垂体素。在本研究中，催产素被定位在子宫内膜的母马在光镜和超微结构水平的免疫染色和免疫金标记的子宫内膜活检标本收集在发情期。强阳性免疫染色催产素被发现在分泌囊泡的分泌（无纤毛）上皮细胞的子宫腔和浅表腺体。用免疫金标记，催产素被检测到分泌上皮细胞的分泌囊泡。囊泡含有免疫反应催产素存在于管腔表面，表明催产素分泌到子宫腔顶端的胞吐。在子宫腔和子宫内膜腺体的纤毛上皮细胞、间质细胞或基底内膜腺体中无阳性免疫染色。据我们所知，这是第一次报告的位置催产素在特定的分泌细胞在子宫内膜的任何国内物种。这种局部合成的子宫催产素可能在母马子宫收缩力和黄体溶解的自分泌/旁分泌控制中发挥重要作用。(C)2003年爱思唯尔科学公司All rights reserved.
It has been reported that oxytocin is produced not only in the hypothalamus and posterior pituitary but also in outside the classical hypothalamo-neurohypophyseal axis such as the ovary, testis, placenta and in some nonreproductive sites. In the mare, oxytocin-mRNA has been identified in the endometrium, and oxytocin and its neurophysin have been identified in the uterus. In the present study, oxytocin was localised in the endometrium of the mare at the light microscopic and ultrastructural level by immunostaining and immunogold labelling of endometrial biopsy specimens collected during estrus.Strong positive immunostaining for oxytocin was found in the secretory vesicles of the secretory (nonciliated) epithelial cells of the uterine lumen and of the superficial glands. Using immunogold labelling, oxytocin was detected in the secretory vesicles of secretory epithelial cells. The vesicles containing immunoreactive oxytocin were present on the luminal surface suggesting that oxytocin is secreted into the uterine lumen by apical exocytosis. There was no positive immunostaining in ciliated epithelial cells of the uterine lumen and endometrial glands, in the stromal cells, or in the basal endometrial glands. To our knowledge, this is the first report of the location of oxytocin in specific secretory cells in the endometrium of any domestic species. This locally synthesised uterine oxytocin may have an important role in the autocrine/paracrine control of uterine contractility and luteolysis in the mare. (C) 2003 Elsevier Science Inc. All rights reserved.