Cell density dependence of focus formation in the C3H/10T1/2 transformation assay.
Cell density dependence of focus formation in the C3H/10T1/2 transformation assay.
复制标题
C3H/10T1/2 转化测定中焦点形成的细胞密度依赖性。
作者：
D. Haber;D. Fox;W. Dynan;W. Thilly
在C3H/10T1/2形态转化实验中，使用苯并(a)芘作为致癌物，研究了体外肿瘤转化的一些动力学。在致癌物处理后的不同时间将细胞分散到新的培养皿中，实验表明，当培养物生长形成融合单层时，潜在转化细胞的比例没有变化，在达到融合后约3周内，潜在转化细胞的比例几乎没有变化，并且在BP治疗后约7周，这一比例迅速增加。当融合苯并(a)芘处理的培养物在开始向焦点形成生长之前分散在新的培养皿中时，在播种的高细胞密度下，转化焦点的形成受到抑制。这种现象与处理后细胞的总分裂次数无关。我们认为形态转化的表型表达依赖于C3H/10T1/2系统中的菌落相互作用，我们尚未了解，但无论是在细胞世代还是绝对时间内，它们都与处理后的时间无关。
Some of the dynamics of neoplastic transformation in vitro have been studied with the use of benzo(a)pyrene as the carcinogen in the C3H/10T1/2 morphological transformation assay. Experiments that involved the dsipersion of cells into new culture dishes at various times after carcinogen treatment have shown that no change in the fraction of potentially transformed cells occurs while cultures grow to form a confluent monolayer, that little or no change in the fraction of potentially transformed cells occurs for approximately 3 weeks after confluence is attained, and that this fraction increases rapidly some 7 weeks after BP treatment. When confluent benzo(a)pyrene-treated cultures are dispersed in new culture dishes prior to the onset of growth toward focus formation, the formation of transformed foci is suppresssed at high cell densities of seeding. This phenomenon is independent of the total number of divisions undergone by cells after treatment. We suggest that phenotypic expression of morphological transformation is dependent on colony interactions in the C3H/10T1/2 system, which we do not yet understand, but which are independent of time posttreatment either in cell generations or absolute time.