Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
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DOI：
10.1016/j.febslet.2010.03.037
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发表时间：
2010-06-03
期刊：
影响因子：
3.5
通讯作者：
Ebizuka, Yutaka
中科院分区：
文献类型：
--
作者：
Shibuya, Masaaki;Nishimura, Kazuya;Ebizuka, Yutaka
三萜皂苷是具有由三萜糖苷配基和糖组成的结构的一组不同的化合物。由于三萜皂苷结构的复杂性，鉴定参与三萜皂苷生物合成的糖转移酶是困难的。鉴定并表征了来自大豆的两种糖基转移酶，命名为GmSGT 2和GmSGT 3。体外分析表明，GmSGT 2将半乳糖基从UDP-半乳糖转移到大豆皂苷元B单葡糖苷酸，GmSGT 3将鼠李糖基从UDP-鼠李糖转移到大豆皂苷III。这些结果表明，大豆皂甙I是由大豆皂甙元醇B通过连续的糖转移反应生物合成的。(C)2010年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Triterpene saponins are a diverse group of compounds with a structure consisting of a triterpene aglycone and sugars. Identification of the sugar-transferase involved in triterpene saponin biosynthesis is difficult due to the structural complexity of triterpene saponin. Two glycosyltransferases from Glycine max, designated as GmSGT2 and GmSGT3, were identified and characterized. In vitro analysis revealed that GmSGT2 transfers a galactosyl group from UDP-galactose to soyasapogenol B monoglucuronide, and that GmSGT3 transfers a rhamnosyl group from UDP-rhamnose to soyasaponin III. These results suggest that soyasaponin I is biosynthesized from soyasapogenol B by successive sugar transfer reactions. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.