Selection based on the folding properties of proteins with ribosome display
Selection based on the folding properties of proteins with ribosome display
复制标题
DOI：
10.1016/s0014-5793(03)00178-9
复制
发表时间：
2003-03-27
期刊：
影响因子：
3.5
通讯作者：
Plückthun, A
中科院分区：
文献类型：
--
作者：
Matsuura, T;Plückthun, A
核糖体展示是通过配体结合选择和进化蛋白质功能的有力工具。在这里，这个体外系统被用来。根据蛋白质的折叠特性进行选择，而不依赖于特定的配体结合。该选择基于错误折叠蛋白质的两个性质：（1）对蛋白质水解的敏感性增加和（2）疏水区域的更大暴露。通过针对这些属性，我们表明，compoundly折叠和可溶性蛋白质可以富集超过不溶性和随机卷曲蛋白质。这种方法对于从随机序列文库中选择和进化折叠蛋白质可能特别有用。(C)2003年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to. perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) increased sensitivity to proteolysis and (2) greater exposure of hydrophobic area. By targeting these properties, we show that compactly folded and soluble proteins can be enriched over insoluble and random coil proteins. This approach may be especially useful for selection and evolution of folded proteins from random sequence libraries. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.