Probiotics promote gut health through stimulation of epithelial innate immunity
Probiotics promote gut health through stimulation of epithelial innate immunity
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DOI：
10.1073/pnas.0910307107
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发表时间：
2010-01-05
影响因子：
11.1
通讯作者：
Cominelli, Fabio
中科院分区：
文献类型：
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作者：
Pagnini, Cristiano;Saeed, Rubina;Cominelli, Fabio
益生菌制剂广泛可用，并具有各种拟议的有益效果，包括促进肠道健康。益生菌在肠道中的作用机制尚不清楚，但通常被归因于抗炎作用。在这里，我们证明了多种益生菌制剂VSL#3通过局部刺激上皮天然免疫反应(即增加上皮源性肿瘤坏死因子-α的产生和体内上皮屏障功能的恢复)来预防肠道炎症的发生。在体外，我们还证明了益生菌能刺激上皮细胞产生肿瘤坏死因子-α并激活核因子-kappaB。我们的结果支持这样的假设，即益生菌通过刺激而不是抑制天然免疫系统来促进肠道健康。此外，我们的发现提供了这样一个观点，即先天免疫缺陷可能在炎症性肠病等肠道疾病的发生和发展中发挥关键作用。
Probiotic formulations are widely available and have a variety of proposed beneficial effects, including promotion of gut health. The mechanisms of action of probiotic bacteria in the intestine are still unclear but are generally attributed to an antiinflammatory effect. Here, we demonstrate that the multiple probiotic formulation VSL#3 prevents the onset of intestinal inflammation by local stimulation of epithelial innate immune responses (i.e., increased production of epithelial-derived TNF-alpha and restoration of epithelial barrier function in vivo). We also demonstrate that probiotic bacteria stimulate epithelial production of TNF-alpha and activate NF-kappa B in vitro. Our results support the hypothesis that probiotics promote gut health through stimulation, rather than suppression, of the innate immune system. Furthermore, our findings provide the perspective that defects in innate immunity may play a critical role in the pathogenesis and progression of intestinal disorders, such as inflammatory bowel disease.