Libraries of composite polyfluors built from fluorescent deoxyribosides
Libraries of composite polyfluors built from fluorescent deoxyribosides
复制标题
DOI：
10.1021/ja027197a
复制
发表时间：
2002-10-02
影响因子：
15
通讯作者：
Kool, ET
中科院分区：
文献类型：
--
作者：
Gao, JM;Strässler, C;Kool, ET
我们报告了一类新型水溶性荧光分子（聚荧光分子），它们由组装在类似 DNA 骨架上的多个单独荧光团组成。合成了四种荧光团脱氧核糖，并在 DNA 合成仪上将这些单独的分子组装成寡聚荧光串。通过聚苯乙烯珠上的拆分和合并方法生成了 256 个四氟化合物的文库。荧光显微镜下的文库图像显示出至少 40−50 种不同的色调和强度。选定的四氟化合物在溶液中以纯形式重新合成，并显示出单个荧光团所不具备的特性，例如大斯托克斯位移。
We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40−50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have.