Glucose-6-phosphate dehydrogenase plays a central role in the response of tomato (Solanum lycopersicum) plants to short and long-term drought
Glucose-6-phosphate dehydrogenase plays a central role in the response of tomato (Solanum lycopersicum) plants to short and long-term drought
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DOI：
10.1016/j.plaphy.2016.04.013
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发表时间：
2016-08-01
影响因子：
6.5
通讯作者：
Esposito, Sergio
中科院分区：
文献类型：
--
作者：
Landi, Simone;Nurcato, Roberta;Esposito, Sergio
本研究旨在调查暴露于短期和长期干旱胁迫的番茄植物（Solanum lycopersicum cv. Red Setter）中磷酸葡萄糖六脱氢酶（G6PDH - EC 1.1.1.49）（氧化戊糖磷酸途径（OPPP）的关键酶）的表达、发生和活性。首次评估了不同生长条件下植物的干旱影响：水培实验室系统，以及受控条件下的温室花盆；有趣的是，观察到的变化似乎与众所周知的胁迫反应机制的诱导密切相关，例如脯氨酸合成的增加、分子伴侣 Hsp70 的积累和抗坏血酸过氧化物酶。结果显示，在干旱时任何栽培系统中生长的植物中，G6PDH 的总活性显着增加，特别是胞浆亚型 (cy-G6PDH) 的表达和出现。有趣的是，结果清楚地表明脱落酸（ABA）途径和信号级联（蛋白磷酸酶2C - PP2C）可能与G6PDH表达、发生和活性的增加密切相关。根据年轻和成年番茄植株在不同持续时间和强度胁迫下提供的一致证据，我们假设G6PDH作为主要还原剂供应商之一具有特定作用，可以抵消干旱胁迫的影响。 (C) 2016 Elsevier Masson SAS。版权所有。
The present study was undertaken to investigate the expression, occurrence and activity of glucose 6 phosphate dehydrogenase (G6PDH - EC 1.1.1.49), the key-enzyme of the Oxidative Pentose Phosphate Pathway (OPPP), in tomato plants (Solanum lycopersicum cv. Red Setter) exposed to short- and long-term drought stress.For the first time, drought effects have been evaluated in plants under different growth conditions: in hydroponic laboratory system, and in greenhouse pots under controlled conditions; and in open field, in order to evaluate drought response in a representative agricultural environment.Interestingly, changes observed appear strictly associated to the induction of well known stress response mechanisms, such as the increase of proline synthesis, accumulation of chaperone Hsp70, and ascorbate peroxidase.Results show significant increase in total activity of G6PDH, and specifically in expression and occurrence of cytosolic isoform (cy-G6PDH) in plants grown in any cultivation system upon drought. Intriguingly, the results clearly suggest that abscissic acid (ABA) pathway and signaling cascade (protein phosphatase 2C - PP2C) could be strictly related to increased G6PDH expression, occurrence and activities.We hypothesized for G6PDH a specific role as one of the main reductants' suppliers to counteract the effects of drought stress, in the light of converging evidences given by young and adult tomato plants under stress of different duration and intensity. (C) 2016 Elsevier Masson SAS. All rights reserved.