Effects of (Sp)- and (Rp)-adenosine cyclic 3',5'-phosphorothioates on electrolyte excretion by the isolated perfused rat kidney.
Effects of (Sp)- and (Rp)-adenosine cyclic 3',5'-phosphorothioates on electrolyte excretion by the isolated perfused rat kidney.
复制标题
(Sp)-和(Rp)-腺苷环3,5-硫代磷酸酯对离体灌注大鼠肾电解质排泄的影响。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Coulson
中科院分区：
文献类型：
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作者：
S. Scheinman;W. Stec;R. Coulson
我们研究了环 AMP 硫代磷酸酯类似物 (cAMPS) 的 (Sp) 和 (Rp) 非对映异构体对离体灌注大鼠肾脏中电解质排泄的影响。 cAMPS 高度渗透穿过管周细胞膜并且不被大鼠肾脏代谢（Coulson 等人，Life Sci.32：1489-1498，1983）。向灌注液中添加 10 microM cAMPS(Sp) 导致显着的磷酸盐尿、碳酸氢盐尿、镁尿和尿钠排泄，并且肾血管阻力或肾小球滤过率没有变化。 cAMPS(Sp) 提高了钙的排泄分数，但比例低于钠的排泄分数，因此钙钠清除率显着降低。 cAMPS(Rp) 10 或 100 microM 对肾电解质排泄没有影响。 (Sp) 非对映异构体的甲状旁腺激素样作用与其已知的激活蛋白激酶的能力一致。
We studied the effects of the (Sp) and (Rp) diastereomers of the phosphorothioate analogue of cyclic AMP (cAMPS) on the excretion of electrolytes in the isolated perfused rat kidney. cAMPS is highly permeant across the peritubular cell membrane and is not metabolized by the rat kidney (Coulson et al., Life Sci. 32: 1489-1498, 1983). Addition of 10 microM cAMPS(Sp) to the perfusate resulted in a significant phosphaturia, bicarbonaturia, magnesuria and natriuresis and no change in renal vascular resistance or glomerular filtration rate. Fractional excretion of calcium was elevated by cAMPS(Sp) but proportionately less than the fractional excretion of sodium so that the ratio of calcium to sodium clearances was significantly lowered. cAMPS(Rp) 10 or 100 microM was without effect on renal electrolyte excretion. The parathyroid hormone-like effects of the (Sp) diastereomer are consistent with its known ability to activate protein kinase.