Analyses of mRNA expression patterns of cohesin Subunits rad21 and rec8 in mice: Germ cell-specific expression of rec8 mRNA in both male and female mice
Analyses of mRNA expression patterns of cohesin Subunits rad21 and rec8 in mice: Germ cell-specific expression of rec8 mRNA in both male and female mice
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DOI：
10.2108/zsj.19.539
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发表时间：
2002-05-01
影响因子：
0.9
通讯作者：
Yamashita, M
中科院分区：
文献类型：
--
作者：
Lee, J;Yokota, T;Yamashita, M
在酵母的有丝分裂和减数分裂中，姐妹染色单体之间的粘合需要一种称为粘合蛋白的多亚基蛋白质复合物。我们在这里研究了酵母有丝分裂粘连蛋白rad21和减数分裂粘连蛋白rec8的小鼠同源物在不同器官中的mRNA表达模式，特别关注它们在性腺中的表达。 Northern 印迹和逆转录聚合酶链反应 (RT-PCR) 分析表明，与 rad21 mRNA 在所有检查器官中普遍表达相反，rec8 仅在性腺中表达。我们进行了原位杂交分析来鉴定性腺中表达 rad21 和 rec8 mRNA 的细胞。在睾丸中，rad21 mRNA在体细胞和精原细胞中表达，但在精母细胞中不表达；相反，rec8 mRNA在精母细胞中表达，但在精原细胞和体细胞中不表达。精子细胞同时表达rad21和rec8 mRNA。在卵巢中，所有卵巢细胞（包括生殖细胞和体细胞）中均检测到了rad21 mRNA，而rec8 mRNA仅在卵母细胞中检测到。因此，与rad21基因的广泛表达不同，rec8的基因表达严格限于雄性小鼠的精母细胞和精细胞以及雌性小鼠的卵母细胞。正如在酵母中所报道的那样，rec8 mRNA 的受限表达模式意味着它在两性哺乳动物的减数分裂中发挥重要作用。我们还基于 Rad21 和 Rec8 的 mRNA 在卵母细胞和精子细胞中共表达的发现，讨论了 Rad21 和 Rec8 的协同功能。
A multisubunit protein complex called cohesin is required for the cohesion between sister chromatids in both mitosis and meiosis in yeast. We investigate here the mRNA expression patterns of mouse homologues of the yeast mitotic cohesin rad21 and the meiotic cohesin rec8 in various organs, with special attention to their expression in gonads. Northern blot and reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that, in contrast to the ubiquitous expression of rad21 mRNA in all of the organs examined, rec8 was expressed only in the gonads. We conducted in situ hybridization analysis to identify the cells that express rad21 and rec8 mRNAs in the gonads. In the testis, rad21 mRNA was expressed in somatic cells and spermatogonia but not in spermatocytes, and conversely, rec8 mRNA was expressed in spermatocytes but not in spermatogonia or somatic cells. Spermatids expressed rad21 and rec8 mRNAs simultaneously. In the ovary, rad21 rnRNA was detected in all of the ovarian cells including germ cells and somatic cells, whereas rec8 mRNA was detected only in oocytes. Unlike the widespread expression of rad21 gene, therefore, the gene expression of rec8 is strictly confined to spermatocytes and spermatids in male mouse and oocytes in female mouse. The restricted expression pattern of rec8 mRNA implies its essential role in meiosis in both sexes of mammals, as has been reported in yeast. We also discuss the cooperative functions of Rad21 and Rec8 on the basis of the finding that their mRNAs are coexpressed in oocytes and spermatids.