Aberrant intracellular localization of RCAS1 is associated with tumor progression of gastric cancer.
Aberrant intracellular localization of RCAS1 is associated with tumor progression of gastric cancer.
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RCAS1 的异常细胞内定位与胃癌的肿瘤进展相关。
DOI：
10.3892/ijo.19.4.695
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发表时间：
2001
影响因子：
5.2
通讯作者：
Takeshi Watanabe
中科院分区：
文献类型：
--
作者：
Masaru Kubokawa;Manabu Nakashima;Takashi Yao;K. Ito;N. Harada;Hajime Nawata;Takeshi Watanabe
RCAS1是一种新的肿瘤相关抗原，在子宫和卵巢癌细胞以及其他哺乳动物癌细胞中表达频率较高。我们研究了RCAS1在胃癌中的表达与临床病理特征的关系。免疫组织化学显示，98.4%的胃癌组织中RCAS1蛋白阳性。而在胃腺瘤(100%)、胃溃疡(66.7%)和正常胃上皮(100%)等非癌胃上皮细胞中也有表达。RCAS1在良、恶性细胞中的表达模式有显著差异。在正常胃粘膜、胃溃疡和胃腺瘤中，RCAS1仅定位于粘膜上皮细胞的核周区(PN模式)，而在大多数胃癌(83.9%)中，RCAS1弥漫分布于肿瘤细胞的胞浆和细胞膜中(DC模式)。半定量逆转录聚合酶链式反应分析显示，胃腺癌组织中RCAS1mRNA水平显著高于非肿瘤组织(p=0.038)。高分化腺癌RCAS1表达的PN型(25%)明显高于中分化腺癌(0%)(p=0.01)。此外，值得注意的是，RCAS1表达的DC模式在侵犯粘膜下层的癌(100%)中比粘膜内癌(67.7%)更常见(p=0.0026)。这些结果表明，RCAS1在细胞内的分布改变与胃癌的进展密切相关，是胃癌诊断和预后的有用指标。
A novel tumor-associated antigen, RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is expressed at a high frequency in human uterine and ovarian cancer cells as well as in other mammalian cancer cells. We investigated a relationship between RCAS1 expression and clinicopathological features in gastric cancer. Immunohistochemically, RCAS1 was detected in 98.4% of gastric carcinomas. However, its expression was also observed in non-cancerous gastric epithelial cells including gastric adenomas (100%), gastric ulcers (66.7%) and normal gastric epithelia (100%). Striking difference was observed in the pattern of RCAS1 expression between benign and malignant cells. In cases of normal gastric mucosae, gastric ulcers and gastric adenomas, RCAS1 was localized only in the perinuclear region of the mucosal epithelial cells (PN pattern), while, in most of gastric cancers (83.9%), it was detected diffusely in the cytoplasm and cell membranes of the tumor cells (DC pattern). In semi-quantitative RT-PCR analysis, RCAS1 mRNA levels in gastric adenocarcinoma tissues were significantly higher than those in non-neoplastic tissues (p=0.038). The PN pattern of RCAS1 expression was more frequently observed in well differentiated adenocarcinoma (25%) than in moderately differentiated adenocarcinoma (0%) (p=0.01). In addition, it is noteworthy that DC pattern of RCAS1 expression was more frequently recognized in carcinomas which invaded beyond the submucosa (100%) compared to intramucosal carcinoma (67.7%) (p=0.0026). These findings suggest that altered intracellular distribution of RCAS1 is strictly associated with tumor progression of gastric cancer and is a useful marker for the diagnosis and prognosis in gastric cancer.