In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract
In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract
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DOI：
10.5511/plantbiotechnology.11.1020a
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Hiratsuka, Kazuyuki
中科院分区：
文献类型：
--
作者：
Minami, Taichi;Tanaka, Tsuneyuki;Hiratsuka, Kazuyuki
以往的研究表明，Housaku Monogatari（HM）是一种由酵母细胞壁提取物制成的植物活化剂，对各种植物病害都有很好的防治效果。为了研究HM处理对植物基因表达的影响，我们利用烟草病程相关蛋白1a（PR-1a）基因启动子和拟南芥植物防御素1.2（PDF1.2）基因启动子-荧光素酶融合体作为报告基因，检测了HM处理对植物防御相关基因表达的诱导作用。用HM处理携带启动子-荧光素酶融合基因的转基因拟南芥植物，并监测启动子活性作为植物中荧光素酶活性的变化。生物发光检测结果表明，HM处理后启动子在不同时间被激活。PR-1a启动子的最大激活发生在治疗后4天，PDF1.2启动子的最大激活发生在治疗后4小时。这些结果表明，HM可能包含多种微生物相关分子模式（MAMPs），激活系统获得性抗性，并在不同的时间诱导系统抗性信号通路。这可能解释了HM处理诱导植物对多种病原菌的防御反应的机制。
Previous studies indicated that Housaku Monogatari (HM), a plant activator made from yeast cell wall extract, is effective for control of various plant diseases. To investigate the effect of HM treatment on plant gene expression, we tested the expression of defense related gene induction by exploiting tobacco pathogenesis-related protein 1a (PR-1a) gene promoter-and the Arabidopsis plant defensin 1.2 (PDF1.2) gene promoter-luciferase fusions as reporter genes. Transgenic Arabidopsis plants harboring promoter-luciferase fusion genes were treated with HM and the promoter activity was monitored as changes in luciferase activity in planta. Results of bioluminescence monitoring assay indicated that the promoters were activated at different times after the treatment of test plants with HM. Maximum activation of the PR-1a promoter occurred 4 days, and of the PDF1.2 promoter 4 h, after treatment. These results suggest that HM might contain multiple microbe-associated molecular patterns (MAMPs) that activate systemic acquired resistance and induced systemic resistance signaling pathways at different times. This may explain the mechanisms involved in the induction of defense responses against multiple plant pathogens by HM treatment.