Divergent regulation of the Glut 1 and Glut 4 glucose transporters in isolated adipocytes from Zucker rats.
Divergent regulation of the Glut 1 and Glut 4 glucose transporters in isolated adipocytes from Zucker rats.
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Zucker 大鼠分离脂肪细胞中 Glut 1 和 Glut 4 葡萄糖转运蛋白的不同调节。
DOI：
10.1172/jci115804
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kahn,BB
中科院分区：
文献类型：
--
作者：
Pedersen,O;Kahn,CR;Kahn,BB
我们研究了瘦肉大鼠（FA/FA）和肥胖大鼠（FA/FA）在5、10和20周龄以及链脲佐菌素诱导急性糖尿病后，葡萄糖摄取率与Glut 1和Glut 4蛋白和mRNA水平的关系。5周肥胖大鼠表现为胰岛素高反应性葡萄糖摄取，而20周肥胖大鼠表现为胰岛素抵抗性葡萄糖摄取。在肥胖大鼠脂肪细胞中，每等量的总RNA和总膜蛋白中，Glut 1和Glut 4 mRNA和蛋白的相对丰度分别较低。然而，在所有年龄段，肥胖大鼠的脂肪细胞的扩大伴随着每个细胞的总RNA和总膜蛋白的数倍增加。因此，在细胞基础上，Glut 4的mRNA和蛋白水平在年轻肥胖大鼠中升高，并随着年龄的增长而逐渐下降。肥胖大鼠脂肪细胞的基础葡萄糖摄取增加了数倍，同时肥胖大鼠每个细胞的Glut - 1表达在所有年龄段都比瘦大鼠增加了两到三倍。20周肥胖大鼠的急性糖尿病导致葡萄糖摄取的严重下调，并伴随Glut 1和Glut 4蛋白水平的降低。因此，在Zucker大鼠肥胖和糖尿病的进化过程中，Glut 4表达的变化是胰岛素刺激的脂肪细胞葡萄糖摄取改变的主要原因。图片
We have studied the relationship between glucose uptake rate and Glut 1 and Glut 4 protein and mRNA levels per fat cell in lean (FA/FA) and obese (fa/fa) Zucker rats at 5, 10, and 20 wk of age, and after induction of acute diabetes with streptozotocin. 5 wk obese rats exhibit insulin hyperresponsive glucose uptake, whereas 20 wk obese rats show insulin resistant glucose uptake. The relative abundance of Glut 1 and Glut 4 mRNA and protein per equal amount of total RNA and total membrane protein, respectively, is lower in adipocytes from obese rats. However, at all ages the enlargement of fat cells from obese rats is accompanied by a severalfold increase in total RNA and total membrane protein per cell. Thus, on a cellular basis, mRNA and protein levels of Glut 4 increases in young obese rats and gradually declines as a function of age. Basal glucose uptake is increased severalfold in fat cells from obese rats, and in parallel Glut 1 expression per cell in obese rats is two- to threefold increased over lean rats at all ages. Acute diabetes in 20 wk obese rats causes a profound downregulation of glucose uptake and a concomitant reduction of both Glut 1 and Glut 4 protein levels. Thus, changes in Glut 4 expression are a major cause of alteration in insulin-stimulated glucose uptake of adipocytes during evolution of obesity and diabetes in Zucker rats.Images