Short-term effect of oestradiol on neurokinin B mRNA expression in the infundibular nucleus of ewes
Short-term effect of oestradiol on neurokinin B mRNA expression in the infundibular nucleus of ewes
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DOI：
10.1046/j.1365-2826.2003.01054.x
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发表时间：
2003-08-01
影响因子：
3.2
通讯作者：
Bruneau, G
中科院分区：
文献类型：
--
作者：
Pillon, D;Caraty, A;Bruneau, G
在绵羊中，基本上所有漏斗核的神经激肽B（NKB）神经元都表达雌二醇受体α，对雌性和雄性大脑的分析揭示了漏斗核中NKB神经元数量的异常显著的雌性主导性别差异。这个神经元群体位于雌二醇敏感的脑区，参与控制促性腺激素释放激素（GnRH）分泌和发情行为，但其生理作用的记录很少。本研究的目的是分析NKB mRNA的表达在一个关键的时间时，类固醇刺激的途径，导致这两个事件的诱导。克隆一个特定的羊NKB反义核糖核酸探针后，我们研究了短期雌二醇治疗（4小时皮下）的NKB mRNA的表达在尾部的漏斗核的炔雌醇引发的卵巢切除母羊的影响。我们证明，雌二醇降低了NKB mRNA表达水平（34%）和含有NKB mRNA的细胞数量（43%）。雌二醇在短期内强烈作用于这些NKB细胞。我们认为，在排卵前期NKB mRNA表达的这种早期变化可能参与了GnRH分泌或发情行为的诱导控制。
In sheep, essentially all the neurokinin B (NKB) neurones of the infundibular nucleus express oestradiol receptor alpha, and analysis of female and male brains has revealed an exceptionally marked female-dominant sex difference in the numbers of NKB neurones in the infundibular nucleus. This neuronal population is located in an oestradiol-sensitive brain area involved in the control of gonadotropin-releasing hormone (GnRH) secretion and oestrous behaviour, but its physiological role is poorly documented. The aim of the present study was to analyse NKB mRNA expression at a crucial time when the steroid has stimulated the pathways leading to the induction of these two events. After cloning a specific ovine NKB antisense riboprobe, we examined the effects of a short oestradiol treatment (4 h subcutaneously) on the expression of NKB mRNA in the caudal part of the infundibular nucleus of progesterone-primed ovariectomized ewes. We demonstrated that oestradiol decreased both the level of NKB mRNA expression (34%) and the number of cells containing NKB mRNA (43%). Oestradiol acts strongly on these NKB cells in the short term. We suggest that this early change in NKB mRNA expression during the preovulatory period might be involved in the control of the induction of GnRH secretion or oestrous behaviour.