Influence of cadmium stress on root exudates of high cadmium accumulating rice line (Oryza sativa L.)
Influence of cadmium stress on root exudates of high cadmium accumulating rice line (Oryza sativa L.)
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DOI：
10.1016/j.ecoenv.2017.12.014
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发表时间：
2018-04-15
影响因子：
6.8
通讯作者：
Zhang, Xizhou
中科院分区：
文献类型：
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作者：
Fu, Huijie;Yu, Haiying;Zhang, Xizhou
采用Lu527-8（高Cd积累品系）和Lu527-4（正常品系）两种不同镉（Cd）积累水稻品系进行水培试验，探讨Cd胁迫、根系分泌物和Cd积累之间的联系。结果表明：（1）Cd胁迫增加了两个水稻品系的有机酸含量，但对根系分泌物的组成没有影响。在Cd处理中，Lu527-8根系分泌物中各检测到的有机酸含量是Lu527-4的1.76-2.43倍。两个水稻品系中有机酸含量与Cd含量均呈显着正相关，只是苹果酸仅与Lu527-8高度相关，而与Lu527-4不高度相关。 (2)Cd胁迫下根系分泌物中氨基酸的组成和数量均发生较大变化,且这种变化在两个水稻品系中存在差异。作为对照，在两个水稻品系中检测到四种氨基酸（谷氨酸、甘氨酸、酪氨酸和组氨酸）。 Cd胁迫下，Lu527-8中可检测到8个氨基酸，Lu527-4中可检测到7个氨基酸，其中苯丙氨酸仅由Lu527-8分泌，两个水稻品系均分泌丙氨酸、蛋氨酸和赖氨酸。 Lu527-8中这4种新分泌氨基酸的含量随Cd剂量的增加而显着增加，且与Cd含量呈高度正相关，而Lu527-4中没有出现同样的变化。两个水稻品系在有机酸和氨基酸分泌方面的差异可能与其不同的镉吸收特性有关。
A hydroponic experiment with two different cadmium (Cd) accumulating rice lines of Lu527-8 (the high Cd accumulating rice line) and Lu527-4 (the normal rice line) was carried out to explore the links among Cd stress, root exudates and Cd accumulation. The results showed that (1) Cd stress increased quantities of organic acids, but had no effect on composition in root exudates of the two rice lines. In Cd treatments, the contents of every detected organic acid in root exudates of Lu527-8 were 1.76-2.43 times higher than those of Lu527-4. Significant positive correlations between organic acids contents and Cd contents in plants were observed in both rice lines, except that malic acid was only highly relevant to Lu527-8, but not to Lu527-4. (2) Both composition and quantities of amino acids in root exudates changed a lot under Cd stress and this change differed in two rice lines. In control, four amino acids (glutamic acid, glycine, tyrosine and histidine) were detected in two rice lines. Under Cd stress, eight amino acids in Lu527-8 and seven amino acids in Lu527-4 could be detected, among which phenylalanine was only secreted by Lu527-8 and alanine, methionine and lysine were secreted by both rice lines. The contents of those four newly secreted amino acids from Lu527-8 increased significantly with the increase of Cd dose and each had a high-positive correlation with Cd contents, but the same change did not appear in Lu527-4. The difference between two rice lines in secretion of organic acids and amino acids may be related to their different Cd uptake properties.