Sensitive and selective ctDNA detection based on functionalized black phosphorus nanosheets
Sensitive and selective ctDNA detection based on functionalized black phosphorus nanosheets
复制标题
基于功能化黑磷纳米片的灵敏选择性ctDNA检测
DOI：
10.1016/j.bios.2020.112384
复制
发表时间：
2020
影响因子：
12.6
通讯作者：
Xue-Feng Yu
中科院分区：
文献类型：
--
作者：
Chi Huang;Shushu Hu;Xue Zhang;Haodong Cui;Lie Wu;Na Yang;Wenhua Zhou;Paul K. Chu;Xue-Feng Yu
循环肿瘤DNA （ctDNA）鉴定是早期肿瘤诊断最有意义的方法之一。然而，有效和实用的方法来分析这类新兴的生物标志物仍然缺乏。在这项工作中，制备了一种基于硝基苯功能化黑磷纳米片（np - bp）的生物传感器，用于灵敏和选择性检测ctDNA。在这项工作中，制备了一种硝基苯功能化黑磷纳米片（np - bp）生物传感器，用于灵敏和选择性地检测ctDNA。由于成功的硝基苯功能化，与双链DNA相比，np - bp生物传感器表现出更高的猝灭效率和对单链DNA （ssDNA）更强的亲和力。因此，当染料标记的ssDNA探针在其特定的ctDNA靶标存在下形成dsDNA时，np - bp生物传感器表现出5.4倍的荧光增强。该生物传感器具有50 fM的检测限和50 fM-80 pM的宽线性检测范围，在短时间（15分钟）内提供可靠的读数。此外，基于np - bps的生物传感器可用于临床血清样品的单核苷酸多态性鉴别。可以预见，基于np - bps的传感平台在癌症早期诊断和监测癌症进展方面具有很大的潜力。
Circulating tumor DNA (ctDNA) identification is one of the most meaningful approaches towards early cancer diagnosis. However, effective and practical methods for analyzing this emerging class of biomarkers are still lacking. In this work, a biosensor based on nitrophenyl functionalized black phosphorus nanosheets (NP-BPs) is fabricated for sensitive and selective detection of ctDNA. In this work, a nitrophenyl functionalized black phosphorus nanosheets (NP-BPs) biosensor is fabricated for sensitive and selective detection of ctDNA. Due to the successful nitrophenyl functionalization, the NP-BPs biosensor shows higher quenching efficiency and stronger affinity towards single-stranded DNA (ssDNA), as compared with double-stranded DNA (dsDNA). Therefore, the NP-BPs biosensor exhibits 5.4-fold fluorescence enhancement when dye-labelled ssDNA probe forms dsDNA in the presence of its specific ctDNA target. This biosensor exhibits a detection limit of 50 fM and a wide linear detection range of 50 fM-80 pM, provides reliable readout in a short time (15 min). Moreover, the NP-BPs-based biosensor could be applied to discriminate single nucleotide polymorphisms in clinical serum samples. It is envisioned that the NP-BPs-based sensing platform has great potentials in early cancer diagnosis and monitoring cancer progression.