Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair
Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair
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间充质干细胞衍生的细胞外囊泡用于角膜伤口修复
DOI：
10.1155/2019/5738510
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发表时间：
2019-12
影响因子：
4.3
通讯作者：
Wang Liqiang
中科院分区：
文献类型：
--
作者：
Tao Hongyan;Chen Xiaoniao;Cao Hongmei;Zheng Lingyue;Li Qian;Zhang Kaiyue;Han Zhibo;Han Zhong-Chao;Guo Zhikun;Li Zongjin;Wang Liqiang
骨髓间充质干细胞（mesenchymal stem cells，MSCs）具有免疫调节功能，可通过减轻炎症反应促进组织再生。当这种修复过程受到免疫紊乱或病理性血管生成的干扰时，角膜上皮伤口愈合的延迟可导致持续的上皮缺陷。干细胞衍生的细胞外囊泡（EV）携带丰富的干细胞生物活性分子，为再生治疗提供了一种替代方法。在这项研究中，我们的目的是调查是否从人胎盘来源的骨髓间充质干细胞（hP-MSCs）的EV可以改善小鼠模型角膜碱损伤。将10 μL PBS中的33.33 μg/μL EV应用于角膜。重复施用三次，每天总共施用100 μg EV（在30 μL PBS中），持续两周。结果表明，hP-MSCs来源的EV具有较好的促角膜上皮细胞增殖、抗炎和抑制角膜上皮细胞凋亡的作用。此外，hP-MSC衍生的EV通过抑制血管生成和炎症来改善小鼠角膜伤口愈合。综上所述，我们目前的数据表明，hP-MSC衍生的EV具有角膜伤口愈合的有益效果，这提供了具有巨大实用价值的替代无细胞疗法。
With the immunoregulation potential, mesenchymal stem cells (MSCs) have been used for tissue regeneration by relieving inflammation in the injured tissues. When this repair process is interfered by immune disorders or pathological angiogenesis, the delays in corneal epithelial wound healing can lead to a persistent epithelial defect. Stem cell-derived extracellular vesicles (EVs), which carry abundant bioactive molecules from stem cells, have provided an alternative to regeneration therapy. In this study, we aimed to investigate if EVs from human placenta-derived MSCs (hP-MSCs) could ameliorate alkali injury of the cornea in the mouse model. 33.33 μg/μL EVs in 10 μL PBS were applied to the cornea. Repeat application three times, and 100 μg EVs (in 30 μL PBS) in total were administrated per day for two weeks. Our results revealed that EVs from hP-MSCs had preferable functions including enhancing proliferation and anti-inflammation and suppressing apoptosis of corneal epithelial cells. Furthermore, hP-MSC-derived EVs ameliorated mouse corneal wound healing by inhibiting angiogenesis and inflammation. Taken together, our current data suggested that hP-MSC-derived EVs have the beneficial effects of corneal wound healing, which provide alternative cell-free therapy with great practical value.
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影响因子：
14
作者：
X. Yao;Yi Liu;Jie Gao;Liang Yang;Duo Mao;C. Stefanitsch;Yang Li;Jun Zhang;L. Ou;D. K
通讯作者：
X. Yao;Yi Liu;Jie Gao;Liang Yang;Duo Mao;C. Stefanitsch;Yang Li;Jun Zhang;L. Ou;D. K
影响因子：
158.5
作者：
Eremina, Vera;Jefferson, J. Ashley;Quaggin, Susan E.
通讯作者：
Quaggin, Susan E.
影响因子：
2.8
作者：
Y. Xiang;Wenchang Zhou;Ping Wang;Hui Yang;F. Gao;Hongbing Xiang;A. Manyande;Yu‐ke Tian;X. Tian
通讯作者：
Y. Xiang;Wenchang Zhou;Ping Wang;Hui Yang;F. Gao;Hongbing Xiang;A. Manyande;Yu‐ke Tian;X. Tian
DOI：
10.1073/pnas.182296499
发表时间：
2002-09-03
影响因子：
11.1
作者：
Jin, KL;Zhu, YH;Greenberg, DA
通讯作者：
Greenberg, DA
影响因子：
17.1
作者：
Yuan，Xiaoyong;Marcano，Daniela C.;Acharya，Ghanashyam
通讯作者：
Acharya，Ghanashyam