Rational design of a thermalresponsive-polymer-switchable FRET system for enhancing the temperature sensitivity of upconversion nanophosphors
Rational design of a thermalresponsive-polymer-switchable FRET system for enhancing the temperature sensitivity of upconversion nanophosphors
复制标题
合理设计热响应聚合物可切换FRET系统以增强上转换纳米荧光粉的温度敏感性
DOI：
10.1039/c4nr02497d
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发表时间：
2014-01-01
期刊：
影响因子：
6.7
通讯作者：
Lin, Hongzhen
中科院分区：
文献类型：
--
作者：
Xiao, Qingbo;Li, Yanfang;Lin, Hongzhen
在这里，我们提出了一个温敏聚合物PNIPAM调制的荧光共振能量转移（FRET）系统，以提高温度敏感性的上转换纳米荧光粉（UCNPs）。通过利用红色/近红外双发射NaLuF 4：Mn 2+，Ln（3+）（Ln（3+）= Yb 3+，Er 3+，Tm 3+）UCNP作为能量供体和Au纳米颗粒作为受体，UCNP的温度分辨率在生理温度范围内从3.1 ℃显著增加到0.9 ℃。在我们的样品中将UCNPs和受体结合成离散的纳米复合材料有利于可逆调节UCNPs的发射强度，从而扩展其在生物传感中的应用范围，特别是用于探测生物组织中局部微环境的动态变化。由于智能聚合物可以可逆地响应各种各样的刺激，我们的实验也可以扩展到局部微环境中的各种外部条件，如pH值，金属离子，葡萄糖和组织特异性酶。
Here we propose a thermoresponsive polymer PNIPAM modulated fluorescence resonance energy transfer (FRET) system to enhance the temperature sensitivity of upconversion nanophosphors (UCNPs). By utilizing red/near-infrared dual emitting NaLuF4: Mn2+, Ln(3+) (Ln(3+) = Yb3+, Er3+, Tm3+) UCNPs as the energy donor and Au nanoparticles as the acceptor, the temperature resolution of the UCNPs is significantly increased from 3.1 degrees C to 0.9 degrees C in the physiological temperature range. Conjugating the UCNPs and acceptors into discrete nanocomposites in our samples facilitates reversible regulation of the emission intensity of UCNPs, which thus would extend their application range in biosensing, especially for probing the dynamic changes of local micro-environments in biological tissues. As there are a broad variety of stimuli to which smart polymers can reversibly respond, our experiments are also extendable to various external conditions in local micro-environments, such as pH values, metal ions, glucose, and tissue-specific enzymes.