Host-dependent differences in abundance, composition and host range of cyanophages from the Red Sea
Host-dependent differences in abundance, composition and host range of cyanophages from the Red Sea
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DOI：
10.1111/1462-2920.12569
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发表时间：
2015-04-01
影响因子：
5.1
通讯作者：
Lindell, Debbie
中科院分区：
文献类型：
--
作者：
Dekel-Bird, Naama P.;Sabehi, Gazalah;Lindell, Debbie
蓝细菌在海洋中与大量的细菌共存，这些细菌会感染它们。虽然有许多研究调查了聚球藻，但对原绿球藻的数据要少得多。此外，对噬藻体的组成知之甚少。在这里，我们研究了在春季和夏季从红海收集的样品中的蓝藻噬菌体的丰度和相对组成。在聚球藻上发现的最大丰度为35000 μ g/ml，在以前发现的范围内，而在原绿球藻上发现的24000 μ g/ml比以前的发现高出约10倍。在所有宿主上均分离到T7样、T4样和未知的细菌，包括许多T4样细菌在强光适应的原绿球藻菌株上，而TIM 5样细菌仅在聚球藻上发现。噬藻体的丰度和组成有很大的差异，发现不同的主机在同一采样日期，以及同一主机在不同的日期，与几个可预测的模式识别。宿主范围分析表明，T7-样和TIM 5-样细菌是相当主机特异性的，而T4-样细菌的主机的宽度与主机类型：那些分离的高光适应原绿球藻是相当多的主机特异性比那些低光适应原绿球藻或聚球藻。这些与宿主相关的差异可能导致海洋中宿主-噬菌体相互作用的复杂性。
Cyanobacteria coexist in the oceans with a wealth of phages that infect them. While numerous studies have investigated Synechococcus phages, much less data are available for Prochlorococcus phages. Furthermore, little is known about cyanophage composition. Here, we examined the abundance and relative composition of cyanophages on six cyanobacterial hosts in samples collected during spring and summer from the Red Sea. Maximal abundances found on Synechococcus of 35000 phages/ml are within ranges found previously, whereas the 24000 phages/ml found on Prochlorococcus are approximately 10-fold higher than previous findings. T7-like, T4-like and unknown' phages were isolated on all hosts, including many T4-like phages on high-light adapted Prochlorococcus strains, whereas TIM5-like phages were found only on Synechococcus. Large differences in cyanophage abundance and composition were found for different hosts on the same sampling date, as well as for the same host on different dates, with few predictable patterns discerned. Host range analyses showed that T7-like and TIM5-like phages were quite host-specific, whereas the breadth of hosts for T4-like phages was related to host type: those isolated on high-light adapted Prochlorococcus were considerably more host-specific than those on low-light adapted Prochlorococcus or Synechococcus. These host-related differences likely contribute to the complexity of host-phage interactions in the oceans.