LYSOPHOSPHATIDYLCHOLINE - A CHEMOTACTIC FACTOR FOR HUMAN-MONOCYTES AND ITS POTENTIAL ROLE IN ATHEROGENESIS
LYSOPHOSPHATIDYLCHOLINE - A CHEMOTACTIC FACTOR FOR HUMAN-MONOCYTES AND ITS POTENTIAL ROLE IN ATHEROGENESIS
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DOI：
10.1073/pnas.85.8.2805
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
STEINBERG, D
中科院分区：
文献类型：
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作者：
QUINN, MT;PARTHASARATHY, S;STEINBERG, D
天然低密度脂蛋白（LDL）不影响单核细胞/巨噬细胞的运动。另一方面，氧化修饰的LDL抑制驻留的腹腔巨噬细胞的运动性，但作为循环的人单核细胞的趋化因子。我们现在表明，溶血磷脂酰胆碱（lyso-PtdCho），这是由磷脂酶A2的活性在LDL氧化过程中产生的，是一种有效的单核细胞趋化因子。它对中性粒细胞或常驻巨噬细胞没有趋化作用。 血小板活化因子在用磷脂酶A2处理后，对单核细胞具有趋化性，而完整因子则没有。合成的1-棕榈酰-lyso-PtdCho显示出与来自氧化LDL的lyso-PtdCho级分相当的趋化活性。结果表明，溶血PtdCho氧化LDL可能有利于招募单核细胞进入动脉壁在早期阶段的动脉粥样硬化。溶血性PtdCho的产生，无论是从LDL本身还是从受损细胞的膜磷脂，都可以在全身的炎症过程中发挥更普遍的作用。
Native low density lipoprotein (LDL) does not affect monocyte/macrophage motility. On the other hand, oxidatively modified LDL inhibits the motility of resident peritoneal macrophages yet acts as a chemotactic factor for circulating human monocytes. We now show that lysophosphatidylcholine (lyso-PtdCho), which is generated by a phospholipase A2 activity during LDL oxidation, is a potent chemotactic factor for monocytes. It is not chemotactic for neutrophils or for resident macrophages. Platelet-activating factor, after treatment with phospholipase A2, becomes chemotactic for monocytes, whereas the intact factor is not. Synthetic 1-palmitoyl-lyso-PtdCho showed chemotactic activity comparable to that of the lyso-PtdCho fraction derived from oxidized LDL. The results suggest that lyso-PtdCho in oxidized LDL may favor recruitment of monocytes into the arterial wall during the early stages of atherogenesis. Generation of lyso-PtdCho, either from LDL itself or from membrane phospholipids of damaged cells, could play a more general role in inflammatory pocesses throughout the body.