Electrospun composite nanofibers with all-trans retinoic acid and MWCNTs-OH against cancer stem cells
Electrospun composite nanofibers with all-trans retinoic acid and MWCNTs-OH against cancer stem cells
复制标题
含有全反式视黄酸和 MWCNT-OH 的静电纺复合纳米纤维对抗癌症干细胞
DOI：
10.1016/j.lfs.2020.118152
复制
发表时间：
2020-10-01
期刊：
影响因子：
6.1
通讯作者：
Ni, Shilei
中科院分区：
文献类型：
--
作者：
Chen, Haijun;Shi, Yue;Ni, Shilei
目的：癌症干细胞（CSC）是肿瘤的来源，在癌症对治疗的抵抗力中发挥着关键作用。为了改进目前针对CSCs的治疗方法，在这项工作中，我们开发了一种包含羟基化长壁碳纳米管（MWCNTs-OH）和全反式视黄酸（ATRA）的电纺聚己内酯（PCL）纳米纤维的新型系统。材料和方法：通过静电纺丝锻造纳米纤维膜，并通过扫描电子显微镜、X射线衍射和拉曼等评估纳米纤维膜的物理和化学性质。研究了纳米纤维膜的特性及其对 CSC 分化和细胞毒性的影响。最后，评估了纳米纤维膜的体内抗肿瘤效果。主要发现：在最佳条件下形成的纳米纤维光滑无珠。具有 MWCNTs-OH 的纳米纤维膜可以在近红外 (NIR) 照射下提高培养基的温度，从而抑制神经胶质瘤干细胞 (GSC) 的活力。同时，添加的ATRA可以进一步诱导GSCs分化，破坏其干性，降低其对热处理的抵抗力。与未进行近红外辐射相比，近红外辐射2分钟后，1、2和3天后，膜使GSC的体外活力分别降低了13.41%、14.83%和26.71%。每日光照3分钟，持续3天后，GSCs的活力仅为22.75%，在体内也观察到类似的结果。 意义：这些结果表明，结合热疗和分化治疗对CSCs具有有效的细胞毒性。如果在手术切除肿瘤后将纳米纤维膜插入该部位，可能会阻碍肿瘤复发。
Aims: Cancer stem cells (CSCs) are the source of tumors and play a key role in the resistance of cancer to therapies. To improve the current therapies against CSCs, in this work we developed a novel system of electrospun polycaprolactone (PCL) nanofibers containing hydroxylated mull-walled carbon nanotubes (MWCNTs-OH) and all-trans retinoic acid (ATRA).Materials and methods: The nanofiber membranes were forged by electrospinning, and the physical and chemical properties of the nanofiber membranes were evaluated by scanning electron microscopy, XRD and Raman etc. The photothermal properties of nanofiber membranes and their effects on CSCs differentiation and cytotoxicity were investigated. Finally, the anti-tumor effect of nanofiber membranes in vivo was evaluated.Key findings: The nanofibers formed under optimal conditions were smooth without beads. The nanofibrous membranes with MWCNTs-OH could increase temperature of the medium under near-infrared (NIR) illumination to suppress the viability of glioma stem cells (GSCs). Meanwhile, the added ATRA could further induce the differentiation of GSCs to destroy their stemness and reduce their resistance to heat treatment. Compared with no NIR irradiation, after 2min NIR irradiation, the membranes reduced the in-vitro viability of GSCs by 13.41%, 14.83%, and 26.71% after 1, 2, and 3 days, respectively. After 3 min daily illumination for 3 days, the viability of GSCs was only 22.75%, and similar results were observed in vivo.Significance: These results showed efficiently cytotoxicity to CSCs by combining heat therapy and differentiation therapy. The nanofiber membranes if inserted at the site after surgical tumor removal, may hinder tumor recurrence.