Overview of mass spectrometry-based metabolomics: opportunities and challenges.
Overview of mass spectrometry-based metabolomics: opportunities and challenges.
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DOI：
10.1007/978-1-4939-1258-2_1
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Djukovic, Danijel
中科院分区：
文献类型：
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作者：
Gowda, G A Nagana;Djukovic, Danijel
在基础科学、生命科学及其他广泛领域的重要应用的推动下，代谢组学领域在过去十年中呈指数级增长。质谱与色谱和核磁共振相结合是分析复杂生物混合物中代谢物质的两种主要分析途径。由于其固有的显着更高的灵敏度和快速的数据采集，MS 在代谢组学领域发挥着越来越主导的作用。由于需要开发简单的方法来诊断和管理众多且广泛的人类疾病，随着仪器、实验方法、软件和数据库的进步，质谱法取得了巨大的发展。为此，代谢组学领域已经远远超出了定性方法和简单的模式识别方法，转向了一系列全球性和有针对性的定量方法，这些方法现在已被常规使用并提供可靠的数据，从而为推论注入了更大的信心。强大的同位素标记和追踪方法已经变得非常流行。新出现的环境电离技术，例如解吸电离和快速蒸发电离，可以实现直接实时 MS 分析以及新的 MS 成像方法。虽然基于MS的代谢组学提供了对代谢途径和通量以及与多种疾病相关的代谢物生物标志物的深入了解，但人们日益认识到生物混合物的极高复杂性强调了许多挑战，包括未知代谢物鉴定、生物标志物验证和实验室间再现性，需要解决这些挑战才能实现基于MS的代谢组学的全部潜力。本章概述了基于质谱的代谢组学领域的现状，强调了机遇和挑战。
The field of metabolomics has witnessed an exponential growth in the last decade driven by important applications spanning a wide range of areas in the basic and life sciences and beyond. Mass spectrometry in combination with chromatography and nuclear magnetic resonance are the two major analytical avenues for the analysis of metabolic species in complex biological mixtures. Owing to its inherent significantly higher sensitivity and fast data acquisition, MS plays an increasingly dominant role in the metabolomics field. Propelled by the need to develop simple methods to diagnose and manage the numerous and widespread human diseases, mass spectrometry has witnessed tremendous growth with advances in instrumentation, experimental methods, software, and databases. In response, the metabolomics field has moved far beyond qualitative methods and simple pattern recognition approaches to a range of global and targeted quantitative approaches that are now routinely used and provide reliable data, which instill greater confidence in the derived inferences. Powerful isotope labeling and tracing methods have become very popular. The newly emerging ambient ionization techniques such as desorption ionization and rapid evaporative ionization have allowed direct MS analysis in real time, as well as new MS imaging approaches. While the MS-based metabolomics has provided insights into metabolic pathways and fluxes, and metabolite biomarkers associated with numerous diseases, the increasing realization of the extremely high complexity of biological mixtures underscores numerous challenges including unknown metabolite identification, biomarker validation, and interlaboratory reproducibility that need to be dealt with for realization of the full potential of MS-based metabolomics. This chapter provides a glimpse at the current status of the mass spectrometry-based metabolomics field highlighting the opportunities and challenges.