Synchronisation of Plasmodium falciparum and P. knowlesi In Vitro Cultures Using a Highly Specific Protein Kinase Inhibitor.
Synchronisation of Plasmodium falciparum and P. knowlesi In Vitro Cultures Using a Highly Specific Protein Kinase Inhibitor.
复制标题
使用高度特异性蛋白激酶抑制剂在体外培养恶性疟原虫和诺氏疟原虫的同步。
DOI：
10.1007/978-1-0716-2189-9_10
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ressurreição M
中科院分区：
文献类型：
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作者：
Ressurreição M
疟原虫培养物的同步化对于研究与疟原虫生命周期的无性血液阶段相关的时间依赖性事件的复杂性至关重要。在这里，我们描述了一个程序，使用ML10，一个高度特异性的寄生虫环GMP依赖性蛋白激酶（PKG）的抑制剂，以达到恶性疟原虫和P.了解无性生殖血液阶段培养物，并获得高水平的被捕集的成熟裂殖子以及活的释放裂殖子。此外，我们还描述了如何使用ML 10来提高P.以及如何推导ML10在其他恶性疟原虫中的半数有效浓度（EC_（50））。恶性疟原虫实验室株和临床分离株。
Synchronisation ofPlasmodiumcultures is essential to investigate the complexities of time-dependent events associated with the asexual blood stage of the malaria parasite life cycle. Here we describe a procedure using ML10, a highly specific inhibitor of the parasite cyclic GMP-dependent protein kinase (PKG), to attain high synchronicity ofPlasmodium falciparumandP. knowlesiasexual blood-stage cultures and to obtain high levels of arrested mature schizonts as well as viable released merozoites. Additionally, we describe how to use ML10 to improve the transfection efficiency ofP. falciparumparasites and also how to derive the half maximal effective concentration (EC50) of ML10 in otherP. falciparumlaboratory lines and clinical isolates.