Content of Flavonol Glucosides and Some Properties of Enzymes Metabolizing the Glucosides in Onion (Flavonoid in Fruits and Vegetables, Part II)

Content of Flavonol Glucosides and Some Properties of Enzymes Metabolizing the Glucosides in Onion (Flavonoid in Fruits and Vegetables, Part II)
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洋葱中黄酮醇葡萄糖苷的含量及葡萄糖苷代谢酶的一些性质(果蔬中的黄酮类化合物,第二部分)

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发表时间:
1996
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影响因子:
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通讯作者:
Masahiro Suzuki
Masahiro Suzuki
中科院分区:
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文献类型:
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作者:
T. Tsushida;Masahiro Suzuki

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对北海道收割的黄、红、白洋葱中的黄酮苷含量进行了测定。7个黄葱品种的平均含量分别为:3,4‘-二-O-β-葡萄糖苷16.8 mg/100g,4’-O-β-葡萄糖苷18.5 mg/100g,3-O-β-葡萄糖苷0.8 mg/100g,异鼠李素-4‘-O-β-葡萄糖苷2.9 mg/100g。红洋葱含有大约3倍于这些糖苷的量,而白洋葱没有检测到黄酮醇的含量。外部鳞片中各黄酮类化合物的含量均高于内部鳞片。洋葱中存在黄酮醇葡萄糖苷酶和UDPG:黄酮醇葡萄糖基转移酶。4‘-O-β-葡萄糖基转移酶、3-O-β-葡萄糖苷酶、3-O-β-葡萄糖苷酶和4’-O-β-葡萄糖苷酶的最适pH分别为6.0、8.0、4.5和7.0。在所有洋葱品种中,Quercetin-4‘-β-
The contents of flavonol glucosides in yellow, red and white onions harvested in Hokkaido were measured. Seven varieties of yellow onion averaged 16.8mg/100g of quercetin-3, 4'-di-O-β-glucoside, 18.5mg/100g of quercetin-4'-O-β-glucoside, 0.8mg/100g of quercetin-3-O-β-glucoside and 2.9 mg/100g of isorhamnetin-4'-O-β-glucoside. The red onion contained about 3-fold the amount of these glucosides while the white onion contained no detectable amounts of flavonols. The content of each flavonoid was higher in the outer scales than in the inner ones. Flavonol glucosidases and UDPG: flavonol glucosyltransferases were detected in the onions. The optimum pH of quercetin-3O-β-glucosyltransferase, 4'-O-β-glucosyltransferase, 3-O-β-glucosidase and 4'-O-β-glucosidase was 6.0, 8.0, 4.5 and 7.0, respectively. In all the onion varieties, the activity of quercetin-4'-β-